Molecular and General Genetics MGG

, Volume 230, Issue 3, pp 345–352

The gene for a halophilic glutamate dehydrogenase sequence, transcription analysis and phylogenetic implications

  • Nadia Benachenhou
  • Giuseppe Baldacci
Article

DOI: 10.1007/BF00280290

Cite this article as:
Benachenhou, N. & Baldacci, G. Molec. Gen. Genet. (1991) 230: 345. doi:10.1007/BF00280290

Summary

We have isolated and sequenced the gene for a putative NADP-dependent glutamate dehydrogenase from the extremely halophilic archaebacterium Halobacterium salinarium. This gene is transcribed as a unique RNA molecule of about 1700 nucleotides. The 5′end of the transcript contains characteristic consensus transcription initiation and promoter sequences observed in halophilic archaebacteria. The encoded polypeptide, with a predicted length of 435 amino acids, shows significant overall homology and conservation of functional domains when compared with different eubacterial and eukaryotic glutamate dehydrogenases. Surprisingly, the archaebacterial protein shares a larger number of identical amino acid residues with homologous polypeptides from higher eukaryotes than with those from unicellular eukaryotes and eubacteria.

Key words

Halobacterium salinarium NADP-dependent glutamate dehydrogenase Halophilism Archaebacteria Molecular evolution 

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Nadia Benachenhou
    • 1
  • Giuseppe Baldacci
    • 2
  1. 1.Institut de Génétique et MicrobiologieUniversité Paris-SudOrsay cedexFrance
  2. 2.Laboratoire de Biologie Moléculaire de la RéplicationUPR 272-CNRSVillejuifFrance

Personalised recommendations