Molecular and Cellular Biochemistry

, Volume 18, Issue 2–3, pp 81–86 | Cite as

Influence of 4′-6-diamidino-2-phenylindole on the secondary structure and template activities of DNA and polydeoxynucleotides

  • Prakash Chandra
  • Boris Mildner
  • Otto Dann
  • Angleika Metz
General Articles


The interaction between 4′-6-Diamidino-2-Phenylindole-hydrochloride (DAPI) and a variety of DNAs and synthetic polydeoxynucleotides was investigated in order to delineate the nucleic acid structural features necessary for binding. The spectra of DAPI-DNA complexes, measured at various DAPI-DNA molar ratios (r), are hypochromic relative to DNA in the region of its maximum absorption. All the curves pass through an isosbestic point at 268 nm. A new maxima appears in the region of 380–392 nm for DAPI-DNA complexes. The magnitude of the peaks in the region are directly proportional to the amount of drug present in the complex.

Studies with various DNA types and synthetic polydeoxynucleotides indicate that the drug preferentially binds to dAT-rich regions of DNA. This was also confirmed by enzymatic studies. The inhibition of template action by DAPI in a purified DNA-polymerase reaction was dependent on the dAT-content of the template. The implication of these data to explain a selective binding of DAPI to mitochondrial DNA have been discussed.


Nucleic Acid Secondary Structure Structural Feature Maximum Absorption DAPI 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Russel, W. C., Newman, C. and Williamson, D. H. 1975. Nature (London) 253, 461–462.Google Scholar
  2. 2.
    Williamson, D. H. and Fennel, D. J. 1975. in Methods in Cell Biology (D. M. Prescott, editor) Vol. XII, 335–351, Academic Press, New York.Google Scholar
  3. 3.
    Chandra, P., Götz, A., Wacker, A., Verini, M. A., Casazza, A. M., Fioretti, A., Arcamone, F. and Ghione, M. 1972. FEBS-Lett. 19, 327–332.Google Scholar
  4. 4.
    Chandra, P., Götz, A., Wacker, A., Zunino, F., Di Marco, A., Verini, M. A., Casazza, A. M., Fioretti, A., Arcamone, F. and Ghione, M. 1972. Hoppe-Seyler's Z. Physiol. Chem. 353, 393–398.Google Scholar
  5. 5.
    Chandra, P., Zunino, F., Götz, A., Wacker, A., Gericke, D., Di Marco, A., Casazza, A. M., Giuliani, F., Thorbeck, R. R., Arcamone, F. and Ghione, M. 1972. Naturwissenschaften 59, 448–455.Google Scholar
  6. 6.
    Chandra, P. 1974. in Topics in Current Chemistry (Medicinal Chemistry), Edt. Boscke, F., Vol. 52, 99–139, Springer-Verlag, Berlin-Heidelberg-New York.Google Scholar
  7. 7.
    Montenay-Garestier, Th. and Helene, C. 1968. Nature (Lond.) 844–845.Google Scholar
  8. 8.
    Montenay-Garestier, Th. and Helene, C. 1971. Biochemistry 10, 300–306.Google Scholar
  9. 9.
    Helene, C. Dimicoli, J. L. and Brun, F. 1971. Biochemistry 10, 3802–3809.Google Scholar
  10. 10.
    Mutai, K., Gruber, B. A. and Leonard, N. J. 1975. J. Am. Chem. Soc. 97:14, 4905–4104.Google Scholar
  11. 11.
    Dann, O., Bergen, G., Demant, E. and Volz, G. 1971. Liebig. Ann. Chem. 749, 68–89.Google Scholar
  12. 12.
    Richardson, C. C. 1966. in Procedures in Nucleic Acid Res. (G. L. Cantoni and D. R. Davies, editors) 263–276, Harper & Row, London.Google Scholar
  13. 13.
    Chandra, P. Zunino, F., Zaccara, A., Wacker, A. and Götz, A. 1972. FEBS-Lett. 23, 145–149.Google Scholar
  14. 14.
    Chandra, P. 1977. in Modulation of host immune resistance in the prevention or treatment of induced neoplasias (M. A. Chirigos, editor) Fogarty International Center Proceedings 28, 359–368.Google Scholar
  15. 15.
    Wakelin, L. P. G. and Waring, M. J. 1976. Biochem. J. 157, 721–740.Google Scholar
  16. 16.
    Müller, W. and Crother, D. M. 1968. J. Mol. Biol. 35, 251–290.Google Scholar
  17. 17.
    Chandra, P., Steel, L., Ebener, U., Woltersdorf, M., Laube, H. and Will, G. 1976. in Progress in Molecular and Subcellular Biology (F. E. Hahn, Editor), vol. 4, 167–226. Springer-Verlag, Berlin-Heidelberg-New York.Google Scholar
  18. 18.
    Berardi, G. Piperno, G. and Fonty, G. 1972. J. Mol. Biol. 65, 173–189.Google Scholar
  19. 19.
    Grossman, L. I., Watson, R. and Vinograd, J. 1973. Proc. Natl. Acad. Sci., U.S.A. 70, 3339–3343.Google Scholar

Copyright information

© Dr. W. Junk b.v. Publishers 1977

Authors and Affiliations

  • Prakash Chandra
    • 1
    • 2
  • Boris Mildner
    • 1
    • 2
  • Otto Dann
    • 1
    • 2
  • Angleika Metz
    • 1
    • 2
  1. 1.Gustav-Embden-Zentrum der Biologischen Chemie, Abteilung für Molekular-BiologieUniversität Frankfurt/MainGermany
  2. 2.Institut für Pharmazie und Lebensmittelchemie der Universität ErlangenW. Germany

Personalised recommendations