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Molecular and Cellular Biochemistry

, Volume 18, Issue 2–3, pp 81–86 | Cite as

Influence of 4′-6-diamidino-2-phenylindole on the secondary structure and template activities of DNA and polydeoxynucleotides

  • Prakash Chandra
  • Boris Mildner
  • Otto Dann
  • Angleika Metz
General Articles

Summary

The interaction between 4′-6-Diamidino-2-Phenylindole-hydrochloride (DAPI) and a variety of DNAs and synthetic polydeoxynucleotides was investigated in order to delineate the nucleic acid structural features necessary for binding. The spectra of DAPI-DNA complexes, measured at various DAPI-DNA molar ratios (r), are hypochromic relative to DNA in the region of its maximum absorption. All the curves pass through an isosbestic point at 268 nm. A new maxima appears in the region of 380–392 nm for DAPI-DNA complexes. The magnitude of the peaks in the region are directly proportional to the amount of drug present in the complex.

Studies with various DNA types and synthetic polydeoxynucleotides indicate that the drug preferentially binds to dAT-rich regions of DNA. This was also confirmed by enzymatic studies. The inhibition of template action by DAPI in a purified DNA-polymerase reaction was dependent on the dAT-content of the template. The implication of these data to explain a selective binding of DAPI to mitochondrial DNA have been discussed.

Keywords

Nucleic Acid Secondary Structure Structural Feature Maximum Absorption DAPI 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dr. W. Junk b.v. Publishers 1977

Authors and Affiliations

  • Prakash Chandra
    • 1
    • 2
  • Boris Mildner
    • 1
    • 2
  • Otto Dann
    • 1
    • 2
  • Angleika Metz
    • 1
    • 2
  1. 1.Gustav-Embden-Zentrum der Biologischen Chemie, Abteilung für Molekular-BiologieUniversität Frankfurt/MainGermany
  2. 2.Institut für Pharmazie und Lebensmittelchemie der Universität ErlangenW. Germany

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