Molecular and General Genetics MGG

, Volume 241, Issue 1–2, pp 97–105 | Cite as

Replication of the promiscuous plasmid pLSI: a region encompassing the minus origin of replication is associated with stable plasmid inheritance

  • Gloria del Solar
  • Gabriela Kramer
  • Sara Ballester
  • Manuel Espinosa
Original Articles


Deletion of a region of the promiscuous plasmid pLS1 encompassing the initiation signals for the synthesis of the plasmid lagging strand led to plasmid instability in Streptococcus pneumoniae and Bacillus subtilis. This defect could not be alleviated by increasing the number of copies (measured as double-stranded plasmid DNA) to levels similar to those of the wild-type plasmid pLS1. Our results indicate that in the vicinity of, or associated with the single-stranded origin region of pLS1 there is a plasmid component involved in its stable inheritance. Homology was found between the DNA gyrase binding site within the par region of plasmid pSC101 and the pLS1 specific recombination site RSR.

Key words

Minus origin Single-strand origin Plasmid stability Rolling circle replication Single-stranded DNA 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Gloria del Solar
    • 1
  • Gabriela Kramer
    • 1
  • Sara Ballester
    • 1
  • Manuel Espinosa
    • 1
  1. 1.Centro de Investigaciones BiológicasCSICVelázquez 144MadridSpain

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