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Molecular and General Genetics MGG

, Volume 241, Issue 1–2, pp 17–25 | Cite as

Identification and characterization of stylar glycoproteins associated with self-incompatibility genes of Japanese pear, Pyrus serotina Rehd

  • Hidenori Sassa
  • Hisashi Hirano
  • Hiroshi Ikehashi
Original Articles

Abstract

Japanese pear (Pyrus serotina Rehd.) exhibits gametophytic self-incompatibility. Following our previous findings that basic ribonucleases in the styles of Japanese pear are associated with self-incompatibility genes (S-RNases), stylar proteins with high pI values were analyzed by two-dimensional gel electrophoresis further to characterize S-RNases. A group of basic proteins of about 30 kDa associated with self-incompatibility genes were identified. These proteins contained sugar chains which reacted with concanavalin A and wheat germ agglutinin, and thus were designated as S-glycoproteins of Japanese pear. The fact that the S-glycoprotein was expressed at a much lower level in a self-compatible mutant than in the original variety suggested a role of S-glycoproteins in mediating self-incompatibility of Japanese pear. Immunoblot analysis indicated that S-glycoproteins are identical to previously identified S-RNases. The S-glycoproteins were predominantly expressed in the style, in the ovary in trace amounts, and not in leaf, pollen or germinated pollen. The N-terminal amino acid sequences of the S-glycoproteins showed homology not only with each other but also with those of the S-allele-associated proteins from plants of the family Solanaceae at levels of about 30–50%.

Key words

Gametophytic self-incompatibility Japanese pear Pyrus serotina Rehd. S-glycoprotein N-terminal amino acid sequence 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Hidenori Sassa
    • 1
  • Hisashi Hirano
    • 2
  • Hiroshi Ikehashi
    • 1
  1. 1.Faculty of HorticultureChiba UniversityMatsudo, ChibaJapan
  2. 2.Department of Molecular BiologyNational Institute of Agrobiological ResourcesKannondai, Tsukuba, IbarakiJapan

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