Molecular and General Genetics MGG

, Volume 241, Issue 5–6, pp 595–601 | Cite as

Isolation of signaling mutants of tomato (Lycopersicon esculentum)

  • Jonathan Lightner
  • Gregory Pearce
  • Clarence A. Ryan
  • John Browse
Original Articles


As a first step towards developing a genetic system for investigating signaling processes in plants, we have developed a screen for signaling mutants deficient in a wound response. We have isolated two mutants of tomato that lack detectable production of proteinase inhibitors induced systemically in leaves by wounding. The mutants are deficient in the induction of both proteinase Inhibitor I and proteinase Inhibitor II but can be induced to respond at near wild-type levels by methyl jasmonate, a known elicitor of inhibitor production in tomato. While completely deficient in systemic production of proteinase inhibitors, both mutants produce some proteinase inhibitor in wounded leaves. This evidence suggests the existence of two signaling pathways, one local and one systemic, that regulate the induction of proteinase inhibitor snythesis in response to wounding.

Key words

Jasmonate Proteinase inhibitors Signaling pathways Systemin Wound response 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jonathan Lightner
    • 1
  • Gregory Pearce
    • 1
  • Clarence A. Ryan
    • 1
  • John Browse
    • 1
  1. 1.Institute of Biological ChemistryWashington State UniversityPullmanUSA

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