Molecular and General Genetics MGG

, Volume 241, Issue 5–6, pp 554–563

Characterization of the terminal inverted repeats and their neighboring tandem repeats in the Chlorella CVK1 virus genome

  • Takashi Yamada
  • Takanobu Higashiyama
Original Articles

DOI: 10.1007/BF00279897

Cite this article as:
Yamada, T. & Higashiyama, T. Molec. Gen. Genet. (1993) 241: 554. doi:10.1007/BF00279897

Abstract

A unique group of large icosahedral viruses that infect a unicellular green alga (Chlorella sp. NC64A) were isolated from freshwater sources in Japan. These viruses contain a linear double-stranded DNA (dsDNA) genome with hairpin ends. A physical map was constructed for the genomic DNA of CVK1 (Chlorella virus isolated in Kyoto, no. 1) by pulsed-field gel electrophoresis of restriction fragments. The nucleotide sequences around both termini of the CVK1 DNA revealed the presence of inverted terminal repeats (ITR) of approximately 1.0 kb. Adjacent to the ITR, unique sequence elements of 10 to 20 by were directly repeated 20 to 30 times in tandem array. Several copies of these repeat elements were deleted in virus mutants that were occasionally generated from Chlorella cells that were in a putative CVK1 carrier state. These repeats might represent a hot spot of rearrangement in the CVK1 genome.

Key words

Chlorella virus Linear dsDNA genome Hairpin terminus Terminal inverted and tandem repeats 

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Takashi Yamada
    • 1
  • Takanobu Higashiyama
    • 1
  1. 1.Faculty of EngineeringHiroshima UniversityHigashi-HiroshimaJapan

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