Diabetologia

, Volume 30, Issue 7, pp 474–480

Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans

  • G. A. Spinas
  • B. S. Hansen
  • S. Linde
  • W. Kastern
  • J. Mølvig
  • T. Mandrup-Poulsen
  • C. A. Dinarello
  • J. H. Nielsen
  • J. Nerup
Originals

DOI: 10.1007/BF00279615

Cite this article as:
Spinas, G.A., Hansen, B.S., Linde, S. et al. Diabetologia (1987) 30: 474. doi:10.1007/BF00279615

Summary

Human crude and recombinant interleukin 1 (IL-1) was found to dose- and time-dependently affect the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Incubation of rat islets with either 0.5 U/ml or 5 U/ml of crude IL-1 for 1 h had no detectable effect on (pro)insulin biosynthesis. After 24 hours of exposure 0.5 U/ml of crude or 0.6 ng/ml of recombinant IL-1 (beta) increased the (pro)insulin biosynthesis by 42% and 58%, respectively, whereas a 10-fold greater concentration of IL-1 decreased the (pro)insulin biosynthesis by 74% and 89%, respectively. The increase in (pro)insulin biosynthesis was accompanied by an increase in total protein biosynthesis indicating a nonspecific stimulatory action of low IL-1 concentrations. In contrast, high IL-1 concentrations caused a more selective decrease of the (pro)insulin biosynthesis when compared to the total protein biosynthesis. In addition, low IL-1 concentrations were found to increase and high concentrations to decrease the relative levels of pre-proinsulin mRNA suggesting that IL-1 may act both at a pre- and post-translational level of insulin biosynthesis.

Key words

Interleukin 1 pancreatic islets preproinsulin mRNA proinsulin biosynthesis Type 1 (insulin-dependent) diabetes mellitus 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • G. A. Spinas
    • 1
  • B. S. Hansen
    • 2
  • S. Linde
    • 2
  • W. Kastern
    • 2
  • J. Mølvig
    • 2
  • T. Mandrup-Poulsen
    • 1
  • C. A. Dinarello
    • 3
  • J. H. Nielsen
    • 2
  • J. Nerup
    • 1
  1. 1.Steno Memorial HospitalDenmark
  2. 2.Hagedorn Research LaboratoryGentofteDenmark
  3. 3.Department of MedicineTufts University School of MedicineBostonUSA

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