Humangenetik

, Volume 18, Issue 1, pp 63–70 | Cite as

A comparison between quinacrine fluorescence banding and 3H-thymidine incorporation patterns in human chromosomes

  • D. Calderon
  • W. Schnedl
Original Investigations
Received:

Summary

Late 3H-thymidine incorporation patterns and quinacrine fluorescence banding patterns were analyzed in metaphase chromosomes prepared from human blood cultures. In general, late-labeling regions correspond to the strongly fluorescent bands in the chromosomes. However, the dully fluorescent secondary constrictions of the chromosomes Nos. 1, 9 and 16 may show late replication in some instances. In contrast, the brilliantly fluorescent distal part of the Y chromosome is not labeled during the latest phase of the DNA replication. In the male X and in the isopycnotic X of the female the labeling pattern also agrees with the quinacrine fluorescence banding. The heteropycnotic X of the female is still more strikingly late-labeling. However, the pattern of its late replication agrees also with the quinacrine fluorescence bands.

Keywords

Blood Culture Human Blood Late Phase Distal Part Banding Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

An aus menschlichen Blutkulturen gewonnenen Chromosomenpräparaten wurden vergleichende Untersuchungen über die späten Replikationsmuster (mittels 3H-Thymidin-Autoradiographie) und die Quinacrin-Fluorescenz-Bänderungsmuster durchgeführt. Im allgemeinen stimmen die spät replizierenden Abschnitte mit den intensiv fluorescierenden Regionen der Chromosomen überein. Allerdings können die nur schwach fluorescierenden sekundären Constrictionen der Chromosomen Nr. 1, 9 und 16 manchmal auch eine späte Replikation zeigen. Auf der anderen Seite wird der stark fluorescierende Abschnitt des Y-Chromosoms in den letzten Phasen der DNS-Replikation nicht mehr markiert. Beim X-Chromosom des Mannes und beim isopyknotischen X der Frau wird ebenfalls eine Übereinstimmung des Spät-Replikationsmusters und der Quinacrin-Bänderung gefunden. Das heteropyknotische X der Frau zeigt eine noch deutlichere Spät-Replikation; das Muster der Silberkörner stimmt aber ebenfalls mit den Quinacrine-Bändern überein.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • D. Calderon
    • 1
  • W. Schnedl
    • 1
  1. 1.Histologisch-Embryologisches InstitutUniversity of ViennaAustria

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