Human Genetics

, Volume 34, Issue 2, pp 199–206 | Cite as

An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes

  • S. E. Bloom
  • C. Goodpasture
Original Investigations


A reliable technique for staining human chromosomal nucleolar organizers (NOR's) with silver solutions is described. The NOR's can be selectively stained dark brown by silver solutions leaving the chromosome arms unstained and available for counterstaining with orcein or Giemsa dyes. Unequivocal identification of chromosome pairs bearing NOR's can be achieved using fluorescent banding techniques followed by silver staining. The silver staining procedure for NOR's was simplified and standardized through control of the chemical and physical conditions during silver impregnation and developing.


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  1. Bartalos, M., Rainer, J. O.: Human chromosome mapping with an ammoniacal silver staining procedure. Acta Genet. med. (Roma) 21, 139–142 (1972)Google Scholar
  2. Black, M. M., Ansley, H. R.: Histone staining with ammoniacal silver. Science 143, 693–695 (1964)PubMedGoogle Scholar
  3. Black, M. M., Ansley, H. R.: Histone specificity revealed by ammoniacal silver staining. J. Histochem. Cytochem. 14, 177–181 (1966)PubMedGoogle Scholar
  4. Black, M. M., Speer, F. D., Lillick, L. C.: Acid-extractable nuclear proteins of cancer cells. I. Staining with ammoniacal silver. J. nat. Cancer Inst. 25, 967–977 (1960)Google Scholar
  5. Bloom, S. E., Buss, E. G.: Ammoniacal silver staining of embryonic chicken cells and chromosomes. Poultry Sci. 48, 1114–1116 (1969)Google Scholar
  6. Denton, T. E., Howell, W. M., Barrett, J. V.: Human nucleolar organizer chromosomes: satellite associations. Chromosoma (Berl.) (in press, 1975)Google Scholar
  7. Gilbert, A. B.: Triple silver impregnation for selective staining of avian nerves. Stain Technol. 40, 301–304 (1965)PubMedGoogle Scholar
  8. Goodpasture, C., Bloom, S. E.: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma (Berl.) 53, 37–50 (1975)Google Scholar
  9. Goodpasture, C., Bloom, S. E., Hsu, T. C., Arrighi, F. E.: Human nucleolus organizers: The satellites or the stalks? Amer. J. hum. Genet. (in press, 1976)Google Scholar
  10. Howell, W. M., Denton, T. E.: An ammoniacal-silver stain technique specific for satellite III DNA regions on human chromosomes. Experientia (Basel) 30, 1364–1366 (1974)Google Scholar
  11. Howell, W. M., Denton, T. E., Diamond, J. R.: Differential staining of the satellite regions of human acrocentric chromosomes. Experientia (Basel) 31, 260–262 (1975)Google Scholar
  12. Neu, R. L., Ortega, C. C., Barg, G. A., Pinto, W., Jr., Gardner, L. I., Howell, W. M., Denton, T. E.: Inclusion of satellites in an 18/21 translocation chromosome revealed by ammoniacalsilver staining (sat-banding) in a case of partial trisomy 18. J. med. Genet. (in press, 1976)Google Scholar
  13. Peters, A.: Experiments on the mechanism of silver staining. I. Impregnation. Quart. J. micr. Sci. 96, 84–102 (1955a)Google Scholar
  14. Peters, A.: Experiments on the mechanism of silver staining. II. Development. Quart. J. micr. Sci. 96, 103–115 (1955b)Google Scholar
  15. Weddell, G., Glees, P.: The early stages in the degeneration of cutaneous nerve fibres. J. Anat. 76, 65–93 (1941)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • S. E. Bloom
    • 1
    • 2
  • C. Goodpasture
    • 1
    • 2
  1. 1.Department of Poultry ScienceCornell UniversityIthacaUSA
  2. 2.Department of BiologyM. D. Anderson Hospital and Tumor InstituteHoustonUSA

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