Archives of Toxicology

, Volume 54, Issue 1, pp 41–52 | Cite as

Timing of ovulation and implantation in the common marmoset, Callithrix jacchus, by monitoring of estrogens and 6β-hydroxypregnanolone in urine

  • Hans Wolfgang Heger
  • Diether Neubert
Original Investigations


Urinary estrogen and 6β-hydroxypregnanolone excretions have been determined in female marmosets by means of HPTLC. The levels observed in cycling females during implantation and abortion are reported. Hormon analysis revealed a characteristic pattern showing cyclic fluctuations of estrogens and progesterone. Estrogen metabolites increase during the follicular phase up to 1 μg/ml, the progesterone metabolite level rises to 1.2 μg/ml urine during the luteal phase. Maximum values of both are superimposed in many cases. Ovulatory cycles can be monitored by following estrogen and progesterone metabolites in urine samples. Thus ovulation and the onset of pregnancy can be estimated with a precision of ± 24 h (or less).

Key words

Estrogen Hydroxypregnanolone Marmoset Ovulation Timing of pregnancy 

















High Performance Thin-Layer Chromatography


sum of main estrogens


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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Hans Wolfgang Heger
    • 1
  • Diether Neubert
    • 1
  1. 1.Institut für Toxikologie und Embryopharmakologie der Freien Universität BerlinBerlin 33

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