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Archives of Microbiology

, Volume 153, Issue 1, pp 38–42 | Cite as

Extracellular polysaccharides of cowpea rhizobia: compositional and functional studies

  • Bina Mody
  • Meena Bindra
  • Vinod Modi
Original Papers

Abstract

Polysaccharides excreted by cowpea Rhizobium strains JLn(c) and RA-1 were mixtures of complex acidic exopolysaccharides and low molecular weight neutral glucans. These polymers were fractionated using gel filtration chromatography. Purified fractions of the acidic heteropolymer reacted with peanut agglutinin to give precipitin bands when subjected to Ouchterlony gel diffusion. The acidic exopolysaccharide was found to contain mainly glucose, galactose, glucuronic acid, mannose and fucose. The non-carbohydrate substituents of the acidic heteropolymer were pyruvate, acetate and uronate which were identified by infrared and proton nuclear magnetic resonance spectroscopy as well as by chemical analysis.

Key words

Cowpea rhizobia Acidic polysaccharide Peanut agglutinin Gel filtration IR and NMR spectroscopy Pyruvate Acetate 

Abbreviations

EPS

Extracellular polysaccharide

YEM

yeast extract mannitol

PNA

peanut agglutination

1H-NMR

proton nuclear magnetic resonance

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Bina Mody
    • 1
    • 2
  • Meena Bindra
    • 1
    • 2
  • Vinod Modi
    • 1
    • 2
  1. 1.Department of Microbiology, Faculty of ScienceM.S. University of BarodaBarodaIndia
  2. 2.Biotechnology Centre, Faculty of ScienceM.S. University of BarodaBarodaIndia

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