Molecular and General Genetics MGG

, Volume 236, Issue 2–3, pp 214–218 | Cite as

Gene SNQ2 of Saccharomyces cerevislae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases

  • Jörg Servos
  • Eckard Haase
  • Martin Brendel


The yeast gene SNQ2 confers hyper-resistance to the mutagens 4-nitroquinoline-N-oxide (4-NQO) and Triaziquone, as well as to the chemicals sulphomethuron methyl and phenanthroline when present in multiple copies in transformants of Saccharomyces cerevisiae. Subcloning and sequencing of a 5.5 kb yeast DNA fragment revealed that SNQ2 has an open reading frame of 4.5 kb. The putative encoded polypeptide of 1501 amino acids has a predicted molecular weight of 169 kDa and has several hydrophobic regions. Northern analysis showed a transcript of 5.5 kb. Haploid cells with a disrupted SNQ2 reading frame are viable. The SNQ2-encoded protein has domains believed to be involved in ATP binding and is likely to be membrane associated. It most probably serves as an ATP-dependent permease.

Key words

Mutagen hyper-resistance 4-nitroquinolineN-oxide Yeast ATP-dependent permease 


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Copyright information

© Springer-Vertag 1993

Authors and Affiliations

  • Jörg Servos
    • 1
  • Eckard Haase
    • 1
  • Martin Brendel
    • 1
  1. 1.Institut für Mikrobiologie der J.W. Goethe-UniversitätFrankfurt/MainGermany

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