Molecular and General Genetics MGG

, Volume 240, Issue 2, pp 302–306

Doc and copia instability in an isogenic Drosophila melanogaster stock

  • Elena G. Pasyukova
  • Sergey V. Nuzhdin
Article

Abstract

A high degree of heterogeneity and an overall increase in number of insertion sites of the mobile elements Doc and copia were revealed in one substock of an isogenic Drosophila melanogaster stock, while in two other substocks the distribution of copia sites was highly homogenous, but that of Doc sites was again heterogenous. We therefore concluded that copia was unstable in one of the substocks and Doc was unstable in all. Doc instability presumably arose earlier than copia instability. Doc and copia transpositions were directly observed in experiments with one substock. An abundance of copia insertions was revealed in the X chromosome where insertions with deleterious effects are exposed to selection in hemizygous condition. The locations of many other mobile elements (mdg1, mdg2, mdg3, mdg4, 297, B104, H.M.S. Beagle, I, P, BS, FB) were found to be conserved in each substock and did not differ between them, indicating that these mobile elements were stable. This homogeneity is a strong argument against any possibility of inadvertent contamination.

Key words

Mobile elements Doc copia Transpositions Drosophila melanogaster 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Belyaeva ES, Pasyukova EG, Gluschkova IV, Iovleva OV, Kaidanov LZ, Gvozdev VA (1989) Hot spots of mdgl locations in chromosome 2 and fitness level in Drosophila melanogaster stocks of common origin. Genetika (Russ) 25:1047–1059Google Scholar
  2. Berg DE, Howe MM (1989) Mobile DNA American Society for Microbiology, Washington DCGoogle Scholar
  3. Biemont C (1992) Population genetics of transposable elements. A Drosophila point of view. Genetica, in pressGoogle Scholar
  4. Biemont C, Aouar A, Arnault A (1987) Genome reshuffling of the copia element in an inbred line of Drosophila melanogaster. Nature 329:742–744Google Scholar
  5. Charlesworth B, Langley CH (1989) The population genetics of Drosophila transposable elements. Annu Rev genet 23:251–287Google Scholar
  6. Driver A, Lacey SF, Cullingfort TE, Mitchelson A, O'Hare K (1989) Structural analysis of Doc transposable elements associated with mutations at the white and suppressor of forked loci of Drosophila melanogaster. Mol Gen Genet 220:49–52Google Scholar
  7. Finnegan DJ (1990) Transposable elements. Drosophila Inf terv 68:371–382Google Scholar
  8. Finnegan DJ, Rubin GM, Young MW, Hogness DS (1978) Repeated gene families in Drosophila. Cold Spring Harbor Symp Quant Biol 42:1053–1060Google Scholar
  9. Georgiev PG, Kisilev SL, Simonova OB, Gerasimova TI (1990) A novel transposition system in Drosophila melanogaster depending on the Stalker mobile genetic element. EMBO J 9:2037–2044Google Scholar
  10. Gerasimova TI, Mizrokhi LJ, Georgiev GP (1984) Transposition bursts in genetically unstable Drosophila melanogaster. Nature 309:714–716Google Scholar
  11. Gvozdev VA, Belyaeva ES, Ilyin YV, Amosova IS, Kaidanov LZ (1981) Selection and transposition of mobile dispersed genes in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol 45:673–685Google Scholar
  12. Ising G, Block K (1981) Derivation — dependent distribution of insertion sites for a Drosophila transposon. Cold Spring Harb Symp Quant Biol 45:527–544Google Scholar
  13. Kaidanov LZ (1980) The analysis of genetic consequences of selection and inbreeding in Drosophila melanogaster. Genetica 52/53:165–181Google Scholar
  14. Kim AI, Belyaeva ES, Aslanian MM (1990) Autonomous transposition of gypsy mobile element and genetic instability in Drosophila melanogaster. Mol Gen Genet 224:303–308Google Scholar
  15. Lindsley DL, Goell EH (1968) Genetic variations of Drosophila melanogaster. Carnegie Inst Wash Publ 672Google Scholar
  16. Mevel-Ninio M, Mariol MS, Gans M (1989) Mobilization of the gypsy and copia retrotransposons in Drosophila melanogaster induces reversion of the ovoD dominant female sterile mutations: molecular analysis of revertant alleles. EMBO J 8:1549–1558Google Scholar
  17. Pasyukova EG, Belyaeva ES, Ilyinskaya LE, Gvozdev VA (1988) Outcross-dependent transpositions of copia-like mobile genetic elements in chromosomes of an inbred Drosophila melanogaster stock. Mol Gen Genet 212:281–286Google Scholar
  18. Yoshihara M, Takasu-Ishikava E, Hotta Y (1991) Independence of excision frequency and transposition frequency of P-element in Drosophila melanogaster. Jap J Genet 66:535–550Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Elena G. Pasyukova
    • 1
  • Sergey V. Nuzhdin
    • 1
  1. 1.Department of Animal Molecular GeneticsInstitute of Molecular GeneticsMoscowRussia

Personalised recommendations