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Molecular and General Genetics MGG

, Volume 240, Issue 2, pp 238–244 | Cite as

Tissue- and stage-specific modulation of RNA editing of the psbF and psbL transcript from spinach plastids — a new regulatory mechanism?

  • Ralph Bock
  • Rudolf Hagemann
  • Hans Kössel
  • Jörg Kudla
Article

Abstract

The psbE operon of spinach chloroplasts, which includes the genes psbE, psbF, psbL and psbJ, encodes two RNA editing sites. One site corresponds to the initiation codon of the psbL transcript, as has been described earlier for the homologous transcript from tobacco, while at a second editing site, newly reported here, an internal phenylalanine codon of the psbF transcript is restored. Both these sites were investigated with respect to the extent of editing in spinach plastids at various developmental stages. The apparent existence of only completely edited transcripts in etioplasts and chloroplasts, indicates that light-induced processes are not acting as determinants in eliciting the editing process. Reduced editing is, however, observed in the psbF and psbL transcript from seeds and roots. This finding suggests that the RNA editing process is differentially down-regulated in leucoplasts and proplastids and that editing may, therefore, function as a regulatory device in lastid gene expression.

Key words

RNA editing Spinach chloroplasts Plastid differentiation Photosynthesis genes 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Ralph Bock
    • 1
  • Rudolf Hagemann
    • 1
  • Hans Kössel
    • 2
  • Jörg Kudla
    • 1
  1. 1.Institut für GenetikMartin-Luther-Universität HalleHalle/S.Germany
  2. 2.Institut für Biologic IIIUniversität FreiburgFreiburgGermany

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