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Molecular and General Genetics MGG

, Volume 239, Issue 3, pp 409–415 | Cite as

The roles of signal peptide and mature protein in RNase (barnase) export from Bacillus subtilis

  • Mario Chen
  • Vasantha Nagarajan
Article

Abstract

Barnase, an extracellular RNAse from Bacillus amyloliquefaciens is secreted post-translationally from B. subtilis. The rate of secretion of barnase from B. subtilis was improved by replacement of the barnase signal peptide with a heterologous signal peptide. However, the barnase signal peptide exported Escherichia coli alkaline phosphatase faster than mature barnase. Heat shock of B. subtilis cells did not significantly alter the export of barnase using the barnase signal peptide. The slow rate of export of barnase from B. subtilis is due to both the signal peptide and the mature protein sequence rather than either alone.

Key words

Bacilus subtilis Post-translational export Heat shock 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Mario Chen
    • 1
  • Vasantha Nagarajan
    • 1
  1. 1.Central Research and Development DivisionE.I. duPont de Nemours CompanyWilmingtonUSA

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