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Diabetologia

, Volume 32, Issue 9, pp 694–699 | Cite as

Effects of insulin on kidney function and sodium excretion in healthy subjects

  • P. Skøtt
  • O. Hother-Nielsen
  • N. E. Bruun
  • J. Giese
  • M. D. Nielsen
  • H. Beck-Nielsen
  • H. -H. Parving
Originals

Summary

Insulin action on kidney function was evaluated in 8 healthy subjects, (mean age 27 years) using the euglycaemic clamp technique. Insulin was infused at rates of 0, 20 and 40 mU·min−1·m−2 over consecutive periods of 120 min resulting in plasma insulin concentrations of 8±2, 29±7 and 66±14 mU/l. The renal clearance of 51Cr-EDTA, lithium, sodium and potassium was determined during the last 90 min of each period. Sodium clearance declined with increasing plasma insulin concentrations (1.3±0.4, 1.0±0.3 and 0.5±0.2 ml·min−1·1.73 m−2, p<0.001), while glomerular filtration rate (108±21, 104±21 and 108±20ml·min−1·1.73 m−2) and lithium clearance (a marker of fluid flow rate from the proximal tubules) 29±5, 29±4 and 30±4 ml·min−1·1.73 m−2) remained unchanged. Calculated proximal tubular reabsorption of sodium and water was unchanged, while calculated distal fractional sodium reabsorption increased (95.5±1.5, 96.4±1.2 and 98.1±0.7%, p<0.001). Potassium clearance and plasma potassium concentration declined, whereas plasma aldosterone and plasma renin concentrations were unchanged. In conclusion, elevation of plasma insulin concentration within the physiological range has a marked antinatriuretic action. This effect is located distally to the proximal renal tubules.

Key words

lithium clearance glomerular filtration rate sodium excretion insulin normal subjects distal renal tubules 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • P. Skøtt
    • 1
    • 2
  • O. Hother-Nielsen
    • 1
  • N. E. Bruun
    • 2
  • J. Giese
    • 2
  • M. D. Nielsen
    • 2
  • H. Beck-Nielsen
    • 1
  • H. -H. Parving
    • 1
  1. 1.Hvidøre HospitalKlampenborg
  2. 2.Department of Clinical Physiology and Nuclear MedicineGlostrup University HospitalCopenhagenDenmark

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