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Plant Cell Reports

, Volume 8, Issue 5, pp 307–311 | Cite as

Isolation, culture, and regeneration of plants from potato protoplasts

  • Heddwyn Jones
  • Angela Karp
  • Michael G. K. Jones
Article

Abstract

A technique is described for the routine isolation of protoplasts from storage parenchyma cells of potato tubers grown in vitro. The protoplasts typically contained many starch grains. On culture, most of the starch grains were metabolised during the first 7 days, after which the cells began to divide. Following further culture, protoplast-derived colonies and calli were obtained, from which shoots and intact plants were regenerated. Cytological study of regenerated plants showed that the majority were octaploid or aneuploid at the octaploid level. This aspect is compared with plants regenerated from mesophyll protoplasts of potato. The use of tuber protoplasts for studies on tissue-specific transient gene expression of chimeric gene constructs, following their introduction into the protoplasts by electroporation, is discussed, together with the uses of tuber protoplasts in fundamental physiological and biochemical studies.

Keywords

Gene Expression Starch Regenerate Plant Potato Tuber Parenchyma Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Heddwyn Jones
    • 1
  • Angela Karp
    • 1
  • Michael G. K. Jones
    • 1
  1. 1.Rothamsted Experimental Station, Biochemistry DepartmentAFRC Institute of Arable Crops ResearchHarpendenUK

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