Humangenetik

, Volume 30, Issue 1, pp 83–90 | Cite as

Sequential Q- and acridine orange-marker technique

  • Norio Niikawa
  • Tadashi Kajii
Short Communications
Received:

Summary

A standardized Q- and acridine orange (AO)-fluorescence dual marker technique was described. It involved preservation of unstained chromosome slides in a vacuum desiccator up to 18 months, Q-staining, destaining, and treatment in Hanks' solution, pH 5.1, at 85°C for 13 min, and acridine organe staining. Q-markers were found at the paracentromeric regions of chromosomes 3 and 4, the short arms and the satellites of the acrocentric chromosomes, while AO-marker spots were on the satellite-stalks of the acrocentrics. The advantage of the dual marker technique was illustrated by the determination of the origin of trisomy 22 in a spontaneous abortus.

Keywords

Internal Medicine Metabolic Disease Acridine Acridine Orange Vacuum Desiccator 
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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Norio Niikawa
    • 1
  • Tadashi Kajii
    • 1
  1. 1.Laboratory of Embryology and CytogeneticsUniversity Clinic of Gynecology and ObstetricsGenevaSwitzerland

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