Plant Cell Reports

, Volume 7, Issue 4, pp 225–228 | Cite as

Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS

  • Reinhard Töpfer
  • Markus Pröls
  • Jozef Schell
  • Hans-Henning Steinbiß
Article

Abstract

The reporter genes for Chloramphenicolacetyltransferase (CAT), Neomycinphosphotransferase-(NPT)-II and β-Glucuronidase (GUS) were compared in transient gene expression experiments in tobacco mesophyll protoplasts. For this purpose, nearly identical chimeric genes controlled by the CaMV 35 S promoter were constructed. The detection level of each system was determined yielding the following order of relative sensitivity: CAT<NPT II<GUS.

Abbreviations

CAT

Chloramphenicolacetyltransferase

NPT II

Neomycinphosphotransferase

GUS

β-Glucuronidase

CaMV

Cauliflower Mosaic Virus

PEG

Polyethylenglycol

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Reinhard Töpfer
    • 1
  • Markus Pröls
    • 1
  • Jozef Schell
    • 1
  • Hans-Henning Steinbiß
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungAbteilung Genetische Grundlagen der Pflanzenzüchtung, EgelspfadKöln 30Federal Republic of Germany

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