Plant Cell Reports

, Volume 8, Issue 12, pp 722–725 | Cite as

Plant regeneration from mesophyll protoplasts of Diplotaxis muralis, a wild crucifer

  • Samir R. Sikdar
  • Soma Sengupta
  • Srabani Das
  • S. K. Sen
Article
Received:
Revised:

Abstract

Mesophyll protoplasts from leaves of aseptically grown shoot tips of Diplotaxis muralis were isolated (6.2–7.1×105 protoplasts/g fresh weight of tissue) using one step enzyme digestion. The protoplasts (71% viability) underwent divisions (4.2+0.1%) on plating in M8PS2 medium and ultimately formed calli with 0.45+0.03% plating efficiency. Plant regeneration could be achieved both through embryogenesis and organogenesis. The efficiency of plant regeneration through organogenesis was 9 times higher than embryogenesis. Forty eight out of 52 plants regenerated so far from 3 independent experiments were normal with respect to fertility and meiotic chromosomal behavior.

Keywords

Independent Experiment Enzyme Digestion Plant Regeneration Mesophyll Protoplast Step Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

6-benzylaminopurine

GA3

Gibberellic acid

A

Kao and Michayluk, 1981

KM

Kao and Michayluk, 1975

MK3

Modified K3

M8P

Modified 8P

MS

Murashige and Skoog, 1962

NAA

1-naphthalene acetic acid

PE

Plating efficiency

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Samir R. Sikdar
    • 1
  • Soma Sengupta
    • 1
  • Srabani Das
    • 1
  • S. K. Sen
    • 1
  1. 1.Plant Molecular and Cellular GeneticsBose InstituteCalcuttaIndia

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