Plant Cell Reports

, Volume 6, Issue 4, pp 291–293 | Cite as

Cryptosin — a new cardenolide in tissue culture and intact plants of Cryptolepis buchanani Roem. & Schult

  • R. Venkateswara
  • K. Sankara Rao
  • C. S. Vaidyanathan
Article

Abstract

A steroidal glycoside of the digoxin type was isolated from fresh leaves and cultured cells of Cryptolepis buchanani Roem & Schult (Asclepiadaceae). Cultures were raised from leaf and shoot. Explants were cultured on MS medium containing 2,4-D, NAA, BA and coconut milk. Metabolically active calluses were proliferated and maintained on Gamborg's B5 medium with 2,4-D and Kn. The new compound, named cryptosin, was characterised by infrared nmr and mass spectroscopy. Cryptosin was also screened for its pharmacological properties and its cardiotonic action was established.

Keywords

Mass Spectroscopy Tissue Culture Glycoside Digoxin Pharmacological Property 

Abbreviations

PVP

Polyvinylpyrrolidone

2,4-D

2,4-Dichlorophenoxyacetic acid

NAA

Naphthaleneacetic acid

BA

Benzyl adenine

Kn

Kinetin

MS

Murashige & Skoog

Na,K-ATPase

Sodium, potassium — sensitive adenosine triphosphate

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • R. Venkateswara
    • 1
  • K. Sankara Rao
    • 1
  • C. S. Vaidyanathan
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia

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