Cryptosin — a new cardenolide in tissue culture and intact plants of Cryptolepis buchanani Roem. & Schult
Abstract
A steroidal glycoside of the digoxin type was isolated from fresh leaves and cultured cells of Cryptolepis buchanani Roem & Schult (Asclepiadaceae). Cultures were raised from leaf and shoot. Explants were cultured on MS medium containing 2,4-D, NAA, BA and coconut milk. Metabolically active calluses were proliferated and maintained on Gamborg's B5 medium with 2,4-D and Kn. The new compound, named cryptosin, was characterised by infrared nmr and mass spectroscopy. Cryptosin was also screened for its pharmacological properties and its cardiotonic action was established.
Keywords
Mass Spectroscopy Tissue Culture Glycoside Digoxin Pharmacological PropertyAbbreviations
- PVP
Polyvinylpyrrolidone
- 2,4-D
2,4-Dichlorophenoxyacetic acid
- NAA
Naphthaleneacetic acid
- BA
Benzyl adenine
- Kn
Kinetin
- MS
Murashige & Skoog
- Na,K-ATPase
Sodium, potassium — sensitive adenosine triphosphate
Preview
Unable to display preview. Download preview PDF.
References
- Brockbank WJ, Lynn KR (1979) Biochem Biophys Acta 578: 13–16Google Scholar
- Enson JM, Seiber JW (1978) J Chromatogr 148: 521–523.Google Scholar
- Gamborg OL, Miller RA, Ojima K (1968) Exptl Cell Res 50: 151–158.Google Scholar
- Haustein KO, Markwardt F, Repke KRH (1970) Eur J Pharm 10: 1–10.Google Scholar
- Jolad SD, Bates RB, Cole JR, Hoffmann JJ, Siahaan TJ, Timmermann BN (1986) Phytochemistry 25: (11) 2581–2590.Google Scholar
- Koike K, Bevelle C, Talapatra K, Cordell GA, Farnsworth NR (1980) Chem Pharm Bull 28: (2) 401–405.Google Scholar
- Murashige T, Skoog F (1962) Physiol Plant 15: 473–498.Google Scholar
- Narendra N, Vekateswara R, Sankara Rao K, Viswamitra MA, Vaidyanathan CS (1986) Acta Cryst (In press).Google Scholar
- Siddiqui S, Hafeez F, Begum S, Siddiqui BS (1987) Phytochemistry 26: (1) 237–241.Google Scholar
- Singh B, Rastogi RB (1969) Ind J Chem 7: 1105–1110.Google Scholar
- Stahl E (1969) In: Stahl E (ed) Thin-layer Chromatography, A laboratory hand book Ed. III: Springer-Verlag, Berlin, pp.341–346.Google Scholar
- Venkateswara R, Sankara Rao K, Vaidyanathan CS (1986) Planta Medica (communicated).Google Scholar
- Watt G (1972) In: A dictionary of the economic products of India, Vol.III, Delhi, pp 624.Google Scholar