Plant Cell Reports

, Volume 6, Issue 4, pp 291–293 | Cite as

Cryptosin — a new cardenolide in tissue culture and intact plants of Cryptolepis buchanani Roem. & Schult

  • R. Venkateswara
  • K. Sankara Rao
  • C. S. Vaidyanathan


A steroidal glycoside of the digoxin type was isolated from fresh leaves and cultured cells of Cryptolepis buchanani Roem & Schult (Asclepiadaceae). Cultures were raised from leaf and shoot. Explants were cultured on MS medium containing 2,4-D, NAA, BA and coconut milk. Metabolically active calluses were proliferated and maintained on Gamborg's B5 medium with 2,4-D and Kn. The new compound, named cryptosin, was characterised by infrared nmr and mass spectroscopy. Cryptosin was also screened for its pharmacological properties and its cardiotonic action was established.


Mass Spectroscopy Tissue Culture Glycoside Digoxin Pharmacological Property 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





2,4-Dichlorophenoxyacetic acid


Naphthaleneacetic acid


Benzyl adenine




Murashige & Skoog


Sodium, potassium — sensitive adenosine triphosphate


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • R. Venkateswara
    • 1
  • K. Sankara Rao
    • 1
  • C. S. Vaidyanathan
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia

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