Molecular and General Genetics MGG

, Volume 163, Issue 3, pp 277–283 | Cite as

A new conditional lethal mutator (dnaQ49) in Escherichia coli K12

  • Takashi Horiuchi
  • Hisaji Maki
  • Mutsuo Sekiguchi


A conditional lethal mutator, dnaQ49, was found in Escherichia coli K12. The dnaQ49 mutation caused stimulation of rifampicin-, nalidixic acid- and streptomycin-resistant mutation frequencies 100 to 2000 fold at 30°C and the frequencies were further increased 50 to 100 fold at 35°C or higher temperatures. Cells carrying dnaQ49 were unable to grown in salt-free L-broth at 44.5°C, and DNA synthesis but not protein synthesis of the cells was suppressed under the restrictive conditions. The dnaQ gene was located at about 5 min on the E. coli linkage map and the order of the genes residing in this region was determined to be on A-dnaE-metD-dnaQ-proA.


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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Takashi Horiuchi
    • 1
  • Hisaji Maki
    • 1
  • Mutsuo Sekiguchi
    • 1
  1. 1.Department of Biology, Faculty of ScienceKyushu University 33FukuokaJapan

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