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Molecular and General Genetics MGG

, Volume 163, Issue 3, pp 229–239 | Cite as

Development of genetic techniques and the genetic map of the yeast Saccharomycopis lipolytica

  • D. Ogrydziak
  • J. Bassel
  • R. Contopoulou
  • R. Mortimer
Article
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Summary

Genetically useful strains of the hydrocarbon-utilizing yeast Saccharomycopsis lipolytica were developed through extensive inbreeding. Spore viability and the percentage of 4-spored asci were increased to the point where tetrad analysis was possible. Procedures for mutant isolation and scoring, maintenance of stocks, mating, sporulation, complementation, tetrad and random spore analysis have been developed for these inbred strains. Sixty seven mutations in fiftyeight genes have been isolated and utilized in mapping studies. Twenty-two cases of linkage have been detected among the 278 gene pairs investigated. Six linkage fragments have been established and a few genes ordered in these fragments. No centromere, linked markers have yet been detected. Evidence for gene conversion, mitotic recombination and diploidization in S. lipolytica is presented.

Keywords

Recombination Ascus Gene Pair Mapping Study Gene Conversion 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • D. Ogrydziak
    • 1
  • J. Bassel
    • 1
  • R. Contopoulou
    • 1
  • R. Mortimer
    • 1
  1. 1.Division of Medical Physics, Donner LaboratoryUniversity of CaliforniaBerkeleyUSA

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