Molecular and General Genetics MGG

, Volume 168, Issue 3, pp 279–291 | Cite as

Fine structure of the 21S ribosomal RNA region on yeast mitochondrial DNA

III. Physical location of mitochondrial genetic markers and the molecular nature of ω
  • Christa Heyting
  • Hilly H. Menke
Article
Received:

Summary

  1. 1.

    We have determined the physical location of mitochondrial genetic markers in the 21S region of yeast mtDNA by genetic analysis of petite mutants whose mtDNA has been physically mapped on the wild-type mtDNA.

     
  2. 2.

    The order of loci, determined in this study, is in agreement with the order deduced from recombination analysis and coretention analysis except for the position of ω+: we conclude that ω+ is located between C321 (RIB-1) and E514 (RIB-3).

     
  3. 3.

    The marker E514 (RIB-3) has been localized on a DNA segment of 3800 bp, and the markers E354, E553 and cs23 (RIB-2) on a DNA segment of 1100 base pairs; both these segments overlap the 21S rRNA cistron. The marker C321 (RIB-1) has been localized within a segment of 240 bp which also overlaps the 21S rRNA cistron, and we infer on the basis of indirect evidence that this marker lies within this cistron.

     
  4. 4.

    In all our rho+ as well as rho- strains there is a one-to-one correlation between the ω+ phenotype, the ability to transmit the ω+ allele and the presence of a mtDNA segment of about 1000 bp long, located between sequences specifying RIB-3 and sequences corresponding to the loci RIB-1 and RIB-2. This segment may be inserted at this same position into ω- mtDNA by recombination.

     
  5. 5.

    The role which the different allelic forms of ω may play in the polarity of recombination is discussed.

     

Keywords

Recombination Genetic Marker Indirect Evidence Physical Location Recombination Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

rRNA

ribosomal RNA

bp

base pairs

21S

region, region around the 21S ribosomal RNA cistron on yeast mtDNA

EthBr

ethidium bromide

tRNA

transfer RNA

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Christa Heyting
    • 1
  • Hilly H. Menke
    • 1
  1. 1.Section for Medical Enzymology and Molecular Biology, Laboratory of BiochemistryUniversity of Amsterdam, Jan Swammerdam InstituteAmsterdamThe Netherlands

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