Applied Microbiology and Biotechnology

, Volume 31, Issue 5–6, pp 592–596 | Cite as

Comparative fermentability of enzymatic and acid hydrolysates of steam-pretreated aspenwood hemicellulose by Pichia stipitis CBS 5776

  • J. Jeffrey Wilson
  • Lise Deschatelets
  • Nora K. Nishikawa
Environmental Microbiology


Enzymatic hydrolysates of hemicellulose from steam-pretreated aspenwood were more fermentable than the acid hydrolysate after rotoevaporation or ethyl acetate extraction treatments to remove acetic acid and sugar- and lignin-degradation products prior to fermentation by Pichia stipitis CBS 5776. Total xylose and xylobiose utilization from 5.0% (w/v) ethyl acetate extracted enzymatic hydrolysate was observed with an ethanol yield of 0.47 g ethanol/g total available substrate and an ethanol production rate of 0.20 g·l-1 per hour in 72 h batch fermentation.


Fermentation Acetic Acid Ethyl Acetate Xylose Hemicellulose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ando S, Arai I, Kiyoto K, Hanai S (1986) Identification of aromatic monomers in steam-exploded poplar and their influences on ethanol fermentation by Saccharomyces cerevisiae. J Ferment Technol 64:567–570Google Scholar
  2. Beck MJ (1986a) Factors affecting efficiency of biomass fermentation to ethanol. Biotechnol Bioeng Symp 17:617–627Google Scholar
  3. Beck MJ (1986b) Effect of intermittent feeding of cellulose hydrolysate to hemicellulose hydrolysate on ethanol yield by Pachysolen tannophilus. Biotechnol Lett 8:513–516Google Scholar
  4. Biely P, Kratky Z, Vrsanska M, Urmanicova D (1980) Induction and inducers of endo-1,4-β-xylanase in the yeast Cryptococcus albidus. Eur J Biochem 108:323–329Google Scholar
  5. Biely P, MacKenzie CR, Puls J, Schneider H (186) Cooperatively of esterases and xylanases in the enzymatic degradation of acetyl cylan. Bio/technology 4:731–733Google Scholar
  6. Brownell HH, Saddler JN (1987) Steam pretreatment of lignocellulosic material for enhanced enzymatic hydrolysis. Biotechnol Bioeng 29:228–235Google Scholar
  7. Burtscher E, Bobleter O, Schwald W, Concin R, Binder H (1987) Chromatographic analysis of biomass reaction products produced by hydrothermolysis of poplar wood. J Chromatogr 390:401–412Google Scholar
  8. Clark TA, Mackie KL (1984) Fermentation inhibitors in wood hydrolysates derived from the softwood pinus radiata. J Chem Technol Biotechnol 34b:101–110Google Scholar
  9. duPreez JC, Bosch M, Prior BA (1986) The fermentation of hexose and pentose sugars by Candida shehatae and Pichia stipitis. Appl Microbiol Biotechnol 23:228–233Google Scholar
  10. Lee H, Biely P, Latta RK, Barbosa MFS, Schneider H (1986) Utilization of xylan by yeasts and its conversion to ethanol by Pichia stipitis strains. Appl Environ Microbiol 52:320–324Google Scholar
  11. Liu H-S, Hsu H-W, Sayler GS (1988) Bioconversion of d-xylose and pretreated oak sawdust to ethanol using Clostridium thermosaccharolyticum by batch and continuous upflow reactors. Biotechnol Prog 4:40–46Google Scholar
  12. Nishikawa NK, Sutcliffe R, Saddler JN (1988) The influence of lignin degradation products on xylose fermentation by Klebsiella pneumoniae. Appl Microbiol Biotechnol 27:549–552Google Scholar
  13. Parekh SR, Parekh RS, Wayman M (1987) Fermentation of wood-derived acid hydrolysates in a batch bioreactor and in a continuous dynamic immobilized cell bioreactor by Pichia stipitis R. Process Biochem 22:85–91Google Scholar
  14. Puls J, Poutanen K, Viikari L (1985) The effect of steaming pretreatment on the biotechnical utilization of wood components. In: Egneus H, Ellegard A (eds) Bioenergy 84, vol. III, Biomass conversion. Elsevier, London, pp 173–180Google Scholar
  15. Schwald W, Saddler JN (1988) A comparison of methods for quantitating the fermentation of wood derived sugars to solvents. Enzyme Microb Technol 10:37–41Google Scholar
  16. Schwald W, Chan M, Brownell HH, Saddler JN (1988) Influence of hemicellulose and lignin on the enzymatic hydrolysis of wood. In: Aubert JP, Beguin P, Millet J (eds) FEMS Symposium no. 43, Biochemistry and genetics of cellulose degradation, Paris Sept. 7–9, 1987. Academic Press, New York, pp 303–314Google Scholar
  17. Skoog K, Hahn-Hägerdal B (1988) Xylose fermentation. Enzyme Microb Technol 10:66–80Google Scholar
  18. Sutcliffe R, Saddler JN (1986) The role of lignin in the adsorption of cellulases during enzymatic treatment of lignocellulosic material. Biotechnol Bioeng Symp 17:749–762Google Scholar
  19. Tan LUL, Yu EKC, Louis-Seize GW, Saddler JN (1987) Inexpensive, rapid procedure for bulk purification of cellulase-free β-1,4-D-xylanase of high specific activity. Biotechnol Bioeng 30:96–100Google Scholar
  20. Tran AV, Chambers RP (1986) Ethanol fermentation of red oak prehydrolysate by the yeast Pichia stipitis CBS 5776. Enzyme Microb Technol 8:439–444Google Scholar
  21. Watson NE, Prior BA, Lategan PM (1984) Factors in acid treated bagasse inhibiting ethanol production from d-xylose by Pachysolen tannophilus. Enzyme Microb Technol 6:451–456Google Scholar
  22. Zyl C van, Prior BA, duPreez JC (1988) Production of ethanol from sugar cane bagasse hemicellulose hydrolysate by Pichia stipitis. Appl Biochem Biotechnol 17:357–369Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. Jeffrey Wilson
    • 1
  • Lise Deschatelets
    • 1
  • Nora K. Nishikawa
    • 1
  1. 1.Biotechnology and Chemistry DepartmentForintek Canada CorporationOttawaCanada

Personalised recommendations