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Applied Microbiology and Biotechnology

, Volume 31, Issue 5–6, pp 547–550 | Cite as

Combined effect of acetic acid, pH and ethanol on intracellular pH of fermenting yeast

  • M. E. Pampulha
  • M. C. Loureiro-Dias
Applied Microbiology

Summary

The internal pH of Saccharomyces cerevisiae IGC 3507 III (a respiratory-deficient mutant) was measured by the distribution of [14C]propionic acid, when the yeast was fermenting glucose at pH 3.5, 4.5 and 5.5 in the presence of several concentrations of acetic acid and ethanol. Good correlation was obtained between fermentation rates and internal pH. For all external pH values tested, the internal pH was 7.0–7.2 in the absence of inhibitors. The addition of acetic acid and/or ethanol resulted in a decrease of fermentation rate together with a drop in internal pH. Internal pH did not depend on the concentration of total external acetic acid but only on the concentration of the undissociated form of the acid. Ethanol potentiated the effect of acetic acid both with respect to inhibition of fermentation and internal acidification.

Keywords

Glucose Acetic Fermentation Acetic Acid Combine Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Cássio F, Leão C, Uden N van (1987) Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae. Appl Environ Microbiol 53:509–513Google Scholar
  2. Guijarro JM, Lagunas R (1984) Saccharomyces cerevisiae does not accumulate ethanol against a concentration gradient. J Bacteriol 160:874–878Google Scholar
  3. Lafon-Lafourcade S, Geneix C, Ribéreau-Gayon P (1984) Inhibition of alcoholic fermentation of grape must by fatty acids produced by yeast and their elimination by yeast ghosts. Appl Environ Microbiol 47:1246–1249Google Scholar
  4. Leão C, Uden N van (1984) Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 774:43–48Google Scholar
  5. Loureiro V, Ferreira HG (1983) On the intracellular accumulation of ethanol in yeast. Biotechnol Bioeng 25:2263–2269Google Scholar
  6. Nagodawithana TW, Steinkraus KH (1976) Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in “rapid fermentation”. Appl Environ Microbiol 31:158–162Google Scholar
  7. Novak M, Strehaiano P, Moreno M, Goma G (1981) Alcoholic fermentation: on the inhibitory effect of ethanol. Biotechnol Bioeng 23:201–211Google Scholar
  8. Pinto I, Cardoso H, Leão C, van Uden N (1989) High enthalpy and low enthalpy death in Saccharomyces cerevisiae induced by acetic acid. Biotechnol Bioeng 33:0000–0000Google Scholar
  9. Rottenberg H (1979) The measurement of membrane potential and ΔpH in cells, organelles and vesicles. In: Fleischer S, Packer L (eds) Methods in enzymology, vol 55. Academic Press, London, pp 547–569Google Scholar
  10. Serrano R (1988) Structure and function of proton translocating ATPase in plasma membranes of plants and fungi. Biochim Biophys Acta 947:1–28Google Scholar
  11. Uden N van (1967) Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Arch Microbiol 58:155–168Google Scholar
  12. Uden N van (1985) Ethanol toxicity and ethanol tolerance in yeasts. In: Tsao GT (ed) Annual reports on fermentation processes, vol 8. Academic Press, London, pp 11–58Google Scholar
  13. Umbreit WW, Burris RH, Stauffer JF (1964) Manometric techniques, 4th edn. Burgess Publishing Company, MinneapolisGoogle Scholar
  14. Viegas CA, Sá-Correia I, Novais JM (1985) Synergistic inhibition of the growth of Saccharomyces bayanus by ethanol and octanoic or decanoic acids. Biotechnol Lett 7:611–614Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. E. Pampulha
    • 1
  • M. C. Loureiro-Dias
    • 1
  1. 1.Laboratory of MicrobiologyGulbenkian Institute of ScienceOeiras CodexPortugal

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