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Molecular and General Genetics MGG

, Volume 125, Issue 3, pp 197–216 | Cite as

Interactions among genes controlling sensitivity to radiation and alkylation in yeast

  • Martin Brendel
  • Robert H. Haynes
Article

Summary

In the simple eucaryote Saccharomyces cerevisiae there are at least three phenotypically distinct classes of mutants sensitive to inactivation by radiations and alkylating agents: class I mutants are sensitive to ultraviolet light and nitrogen mustard (HN2); class II mutants are sensitive to X-rays and methylmethane sulphonate (MMS); and class III mutants are sensitive to all four of these agents. We have constructed doubly mutant strains of types (I, I), (I, II), (I, III), and (II, III) and have measured their sensitivity to UV, X-rays, HN2 and MMS in order to characterize the interactions of the various mutant gene pairs. Class (I, III) double mutants proved to be supersensitive to UV and HN2 and class (II, III) double mutants proved to be supersensitive to X-rays and MMS. All other double mutants showed little or no enhancement of sensitivity over their most sensitive single mutant parents. Mutants of class I are known to be defective in excision repair and our results are consistent with the idea that there exist at least two additional pathways for dark repair in yeast, one capable of repairing X-ray and MMS damage to DNA, and another, possibly analogous to post-replication repair in bacteria, that competes with the other two for damaged regions in DNA.

Keywords

Saccharomyces Cerevisiae Alkylation Saccharomyces Mutant Strain Double Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Averbeck, D., Laskowski, W., Eckhardt, F., Lehmann-Brauns, E.: Four radiation-sensitive mutants of Saccharomyces. Molec. gen. Genet. 107, 117–127 (1970)Google Scholar
  2. Beam, C. A., Mortimer, R. K., Wolfe, R. G., Tobias, C. A.: The relation of radioresistance to budding in Saccharomyces cerevisiae. Arch. Biochem. Biophys. 49, 110–122 (1954)Google Scholar
  3. Brendel, M., Khan, N. A., Haynes, R. H.: Common steps in the repair of alkylation and radiation damage in yeast. Molec. gen. Genet. 106, 289–295 (1970)Google Scholar
  4. Cox, B. S., Parry, J. M.: The isolation, genetics and survival characteristics of ultraviolet sensitive mutants in yeast. Mutation Res. 6, 37–55 (1968)Google Scholar
  5. Game, J. C., Cox, B. S.: Allelism tests of mutants affecting sensitivity to radiation in yeast and a proposed nomenclature. Mutation Res. 12, 328–331 (1971)Google Scholar
  6. Game, J. C., Cox, B. S.: Epistatic interactions between four Rad loci in yeast. Mutation Res. 16, 353–362 (1972)Google Scholar
  7. Hatchard, C. G., Parker, C. A.: A new sensitive chemical actinometer. II. Potassium ferrioxalate as a standard chemical actinometer. Proc. roy. Soc. A 235, 518–536 (1956)Google Scholar
  8. Haynes, R. H.: Role of DNA repair mechanisms in microbial inactivation and recovery phenomena. Photochem. Photobiol. 3, 429–450 (1964)Google Scholar
  9. Haynes, R. H.: The interpretation of microbial inactivation and recovery phenomena. Radiation Res., Suppl. 6, 1–29 (1966)Google Scholar
  10. Haynes, R. H., Inch, W. R.: Synergistic action of nitrogen mustard and radiation in microorganisms. Proc. nat. Acad. Sci. (Wash.) 50, 839–846 (1963)Google Scholar
  11. Howard-Flanders, P., Boyce, R. P.: DNA repair and genetic recombination: Studies on mutants of Escherichia coli defective in these processes. Radiat. Res., Suppl. 6, 156–184 (1966)Google Scholar
  12. Howard-Flanders, P., Theriot, L., Stedeford, J. B.: Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12. J. Bact. 97, 1134–1141 (1969)Google Scholar
  13. Johnson, J. R., Mortimer, R. K.: Use of snail digestive juice in isolation of yeast tetrads. J. Bact. 78, 292 (1959)Google Scholar
  14. Khan, N. A., Brendel, M., Haynes, R. H.: Supersensitive double mutants in yeast. Molec. gen. Genet. 107, 376–378 (1970)Google Scholar
  15. Lemontt, J. F.: Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68, 21–33 (1971)Google Scholar
  16. Nakai, S., Matsumoto, S.: Two types of radiation-sensitive mutants in yeast. Mutation Res. 4, 129–136 (1967)Google Scholar
  17. Puglisi, P. P.: Genetic control of radiation sensitivity in yeast. Radiat. Res. 31, 856–866 (1967)Google Scholar
  18. Resnick, M. A.: Genetic control of radiation sensitivity in Saccharomyces cerevisiae. Genetics 62, 519–531 (1969)Google Scholar
  19. Resnick, M. A., Setlow, J. K.: Repair of pyrimidine dimer damage induced in yeast by ultraviolet light. J. Bact. 109, 979–987 (1972)Google Scholar
  20. Rörsch, A., Putte, P. van de, Mattern, J. E., Zwenk, H., Sluis, C. A. van: Genetic and enzymatic control of radiation sensitivity in Escherichia coli. In: General aspects of radio-sensitivity: Mechanisms of repair, p. 105–129. Vienna: International Atomic Energy Agency 1966Google Scholar
  21. Snow, R.: Mutants of yeast sensitive to ultraviolet light. J. Bact. 94, 571–575 (1967)Google Scholar
  22. Strauss, B. S.: DNA repair mechanisms and their relation to mutation and recombination. In: Current topics in microbiology and immunology 44, 1–185 (1968)Google Scholar
  23. Unrau, P., Wheatcroft, R., Cox, B. S.: The excision of pyrimidine dimers from DNA of ultraviolet irradiated yeast. Molec. gen. Genet. 113, 359–362 (1971)Google Scholar
  24. Willets, N. S., Clark, A. J.: Characteristics of some multiply recombination-deficient strains of Escherichia coli. J. Bact. 100, 231–239 (1969)Google Scholar
  25. Winkler, U., Johns, H. E., Kellenberger, E.: Comparative study of some properties of bacbacteriophage T4D irradiated with monochromatic ultraviolet light. Virology 18, 343–358 (1962)Google Scholar
  26. Zakharov, I. A., Kozina, T. N., Federova, I. V.: Effets de mutation vers la sensibilitée du rayonnement ultraviolet chez la levure. Mutation Res. 9, 31–39 (1970)Google Scholar
  27. Zimmerman, F. K.: Sensitivity to methylmethanesulfonate and nitrous acid of ultraviolet light-sensitive mutants in Saccharomyces cerevisiae. Molec. gen. Genet. 102, 247–256 (1968)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Martin Brendel
    • 1
    • 2
  • Robert H. Haynes
    • 1
  1. 1.Department of BiologyYork UniversityTorontoCanada
  2. 2.Arbeitsgruppe Mikrobengenetik im Fachbereich BiologieJ. W. Goethe-UniversitätFrankfurt a. M.Federal Republic of Germany

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