Plant Cell Reports

, Volume 1, Issue 5, pp 205–208 | Cite as

Use of isopropyl-N(3-chlorophenyl) carbamate (CIPC) to produce partial haploid cells from suspension cultures of soybean (Glycine max)

  • E. Jill Roth
  • G. Weber
  • K. G. Lark
Article
Received:

Abstract

Suspension cultures of a heterozygous line of soybean (Glycine max) were treated with CIPC. Surviving clones had lost 10–15 chromosomes. A recessive phenotype (non-fluorescence) not expressed in the heterozygote, was expressed with high frequency (15–20%) in these clones. Nucleoside auxotrophs were isolated from the segregant clones consistent with partial haploidy. A selective screen for nucleoside auxotrophs is described.

Keywords

Glycine Nucleoside Suspension Culture Carbamate Haploid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag GmbH & Co. KG 1982

Authors and Affiliations

  • E. Jill Roth
    • 1
  • G. Weber
    • 1
  • K. G. Lark
    • 1
  1. 1.Department of BiologyUniversity of UtahSalt Lake CityUSA

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