Plant Cell Reports

, Volume 3, Issue 1, pp 27–29 | Cite as

Plant regeneration of Oncidium varicosum (Orchidaceae) by means of root tip culture

  • Gilberto B. Kerbauy
Article

Abstract

The effects of an auxin (NAA) and a cyto kinin (BA), as well as those of peptone, thiamin and coconut water (CW) on callus and protocorm-like body (PLB) induction from root tips of Oncidium varicosum grown in vitro were studied. Relatively high concentrations of NAA favoured callus induction. PLBs were obtained from callus culture either in medium with 15% CW+1.25 mg/1 NAA or in the presence of CW alone. The low regeneration capac ity of buds presented by the calli seems to be the result of an incomplete dedifferentiation of their cells. This seems to keep the callus in a “root state”. The apparent presence of high cytokinin levels either in the explant or in the callus may be related to these processes.

Abbreviations

BA

benzyladenine

NAA

naphthaleneacetic acid

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References

  1. Churchill ME, Ball EA, Arditti J (1972) Am Orchid Soc Bull 41: 726–730.Google Scholar
  2. Kerbauy GB (1981) Aspectos citológicos e fisiológicos de tecidos e calos de Nicotiana tabacum L. CV. Wisconsin 38. (PhD Dissertation). Dept of Botany-USP, S.P. Brazil.Google Scholar
  3. Kerbauy GB (1984) Z. Pflanzenphysiol. (in press).Google Scholar
  4. Knudson L (1946) Am Orchid Soc. Bull 15: 214–217.Google Scholar
  5. Peters JA (1981) Autotrofia para citocininas em cultura de tecido de plantas haplóides de Nicotiana tabacum L. CV. W-38 (PhD Dissertation). Dept of Botany-USP, S.P. Brazil.Google Scholar
  6. Robbins WJ, Bartley MA (1937) Science 85: 246–247.Google Scholar
  7. Stetler DA, Laetsch WM (1965) Science 149: 1387–1388.Google Scholar
  8. Stewart J, Button J (1978) Am Orchid Soc Bull 47: 607–612.Google Scholar
  9. Tanaka MY, Senda Y, Hasegawa A (1976) Am Orchid Soc Bull 45: 1022–1024.Google Scholar
  10. Torrey JG (1976) Ann Rev Plant Physiol 27: 435–459.Google Scholar
  11. Vacin EF, Went FW (1949) Bot Gaz 110: 605–613.Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Gilberto B. Kerbauy
    • 1
  1. 1.Department of BotanyUniversity of São PauloSão PauloBrazil

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