Plant Cell Reports

, Volume 7, Issue 2, pp 83–87 | Cite as

The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts

  • Eric B. Swanson
  • Marc P. Coumans
  • Gerry L. Brown
  • Jayanti D. Patel
  • W. D. Beversdorf


Brassica napus L.(cv Topas) plants tolerant to chlorsulfuron (CS) were isolated after selection experiments utilizing microspores and haploid protoplasts. The first microspore-derived plant (M-37,) was CS tolerant, haploid and sterile. Normal plant morphology and fertility was restored after colchicine doubling. A CS tolerant plant was also selected from protoplasts (P-26) isolated from microspore-derived embryo tissue and grown on medium containing CS. P-26 was aneuploid, CS tolerant and had very low fertility. The two selected lines produced selfed progeny which were tolerant to from 10–100 times the CS levels of the corresponding Topas plants. Microspores and protoplasts derived from the selfed plants were also CS tolerant. The segregation pattern for CS tolerance from reciprocally crossed progeny of M-37 and Topas was consistent with a semi-dominant nuclear mode of inheritance. Biochemical analysis of the two mutants indicated that the microspore-derived mutant and F1 crosses contained an altered acetohydroxyacid synthase (AHAS) enzyme, while the AHAS activity of the protoplast mutant was similar to Topas. Selfed seed from the M-37 plants have provided tolerance to CS in both greenhouse and field tests. S1 plants from a second microspore selected mutant (M-42) have tolerated 30 g/ha of CS in greenhouse tests. The two single-celled selection systems are discussed and the microspore selection system highlighted as a new method for in vitro selection.


Colchicine Selection System Selected Mutant Chlorsulfuron Selfed Progeny 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barsby TL, Yarrow SA, Shepard JF (1986) Plant Cell Reports 5:101–103Google Scholar
  2. Cardy B (1986) PhD Thesis, Univ. of Guelph, Guelph, Ontario, CanadaGoogle Scholar
  3. Chaleff RS, Ray TB (1984) Science 223: 1148–1151Google Scholar
  4. Choung PV, Beversdorf WD (1985) Plant Science 39:219–226Google Scholar
  5. Choung PV, Pauls KP, Beversdorf WD (1986) Plant Cell Reports 4:4–6Google Scholar
  6. D'Amato F (1984) Critical Rev in Plant Science 3:73–112Google Scholar
  7. Falco SC, Dumas KS (1985) Genetics 109:21–35Google Scholar
  8. Gamborg OL, Miller RA, Ojima L (1968) Exp Cell Res 50:151–158Google Scholar
  9. Glimelius K (1984) Physiol Plant 61:38–44Google Scholar
  10. Haughn GW, Sommerville C (1986) Mol Gen Genet 204:430–434Google Scholar
  11. Gressel J (1978) Outlook on Agriculture 9 (6):283–287Google Scholar
  12. Gressel J (1985) Weed Physiology 2:159–189Google Scholar
  13. Larkin PJ, Scowcroft WR (1981) Theor Appl Genet 60:197–214Google Scholar
  14. Lichter R (1985) in H. Sorensen (Ed) Advances in the production and utilization of Cruciferous crops Martinus Mijhoff/Dr W Junk Pub: 268–277Google Scholar
  15. Meins F (1983) Ann Rev Plant Physiol 34:327–346Google Scholar
  16. Murishige T, Skoog F, (1962) Physiol Plant 15:473–497Google Scholar
  17. Orton TJ, Browers MA (1985) Theor Appl Genet 69:637–643Google Scholar
  18. Sebastian SA and Chaleff RS (1987) Crop Sci In PressGoogle Scholar
  19. Shaffeek AP (1986) Agdex 606–1:Guide to crop protection in Alberta, Alberta Ariculture, Edmonton, Alberta, CanadaGoogle Scholar
  20. Smith AE, Hsiao AI (1985) Weed Science 33:555–557Google Scholar
  21. Swanson EB, Coumans MP, Mu S-C, Barsby TL, Beversdorf WD (1987) Plant Cell Reports 6:94–97Google Scholar
  22. Wondimagegnehu M, Foy CL (1985) Weed Science 33:564–568Google Scholar
  23. Xu ZH, Davey MR, Cocking EC (1982) Plant Sci Lett 24:117–121Google Scholar
  24. Yadav N, Mcdevitt RE, Benard S, Falco SC (1986) Proc Natl Acad Sci USA 83:4418–4422Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Eric B. Swanson
    • 1
  • Marc P. Coumans
    • 1
  • Gerry L. Brown
    • 1
  • Jayanti D. Patel
    • 1
  • W. D. Beversdorf
    • 1
  1. 1.Department of Plant BiologyAllelix Inc.MississaugaCanada

Personalised recommendations