Plant Cell Reports

, Volume 2, Issue 4, pp 205–208 | Cite as

The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of solanum laciniatum

  • S. F. Chandler
  • J. H. Dodds


In leaf derived callus cultures of Solanum laciniatum Ait. both phenolics and solasodine concentrations increased when medium phosphate or nitrogen concs. were reduced to one-eighth or when sucrose concentration was increased from 3 to 4–8 %. Under these conditions growth was reduced and final FW:DW fell. Growth was inhibited by sucrose depletion and nitrogen supple -mentation. On additional nitrogen the concentrations of phenolics and protein significantly increased, FW:DW was reduced and solasodine concentration was unaffected. In seedling derived cultures phosphate depletion resulted in a significant increase in phenolics concentration, an inhibition of growth and a rise in solasodine concentration.


Nitrogen Phosphate Sucrose Condition Growth Nitrogen Concs 
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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. F. Chandler
    • 1
  • J. H. Dodds
    • 2
  1. 1.Department of Plant BiologyUniversity of BirminghamBirminghamUK
  2. 2.Centro de Investigaciòn y de Estudios Avanzados del I.P.N.Unidad IrapuatoIrapuato, Gt.Mexico

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