Plant Cell Reports

, Volume 8, Issue 10, pp 617–620 | Cite as

Increased capsaicin content in PFP-resistant cells of chili pepper (Capsicum annuum L.)

  • R. Salgado-Garciglia
  • N. Ochoa-Alejo
Article

Abstract

Cell suspensions of chili pepper (Capsicum annuum L.) were subjected to a selection process on semisolid medium containing the amino acid analog p-fluorophenylalanine (PFP). Four cell lines with different degrees of resistance were selected and suspension cultures were established from each of them. Resistance was retained even after 75 days of culture in the absence of PFP. PFP-resistant cell lines accumu lated higher levels of capsaicin than sensitive lines even after prolonged culture in PFP-free medium. Capsaicin production in non-selected cells was only 26.8% of that found in one cell line resistant to 500 μM PFP. The capsaicin content in the non-selected cell suspension and in one of the resis tant cell lines was 6.7% and 24.9% respectively, that of fruits.

Abbreviations

BA

benzyladenine

2,4-D

2,4-dichlorophenoxyacetic acid

PFP

p-fluorophenylalanine

d. wt.

dry weight

f. wt.

fresh weight

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ben-Hayyim G, Kochba J (1983) Plant Physiol 72: 685–690Google Scholar
  2. Bennett D J, Kirby G W (1968) J Chem Soc (C): 442–446Google Scholar
  3. Berlin J (1968) Z Pflanzenphysiol 97: 317–324Google Scholar
  4. Berlin J, Vollmer B (1979) Z Naturforsch 34c: 770–775Google Scholar
  5. Constabel F (1988) Biochem Cell Biol 66: 658–664Google Scholar
  6. Flick CE, Jensen RA, Evans DA (1981) Z Pflanzenphysiol 103: 239–245Google Scholar
  7. Gathercole RWE, Street HE (1976) New Phytol 77: 29–41Google Scholar
  8. Gathercole RWE, Street HE (1978) Z Pflanzenphysiol 89: 283–287Google Scholar
  9. Hall RD, Holden MA, Yeoman MM (1987) Plant Cell, Tissue and Organ Culture 8: 163–176Google Scholar
  10. Iwai K, Suzuki T, Fujiwake H (1979) Agric Biol Chem 43: 2493–2498Google Scholar
  11. Leete E, Louden MCL (1968) J Am Chem Soc 90: 6837–6841Google Scholar
  12. Lindsey K (1985) Planta 165: 126–133Google Scholar
  13. Lindsey K, Yeoman MM (1984a) Planta 162: 495–501Google Scholar
  14. Lindsey K, Yeoman MM (1984b) J Exp Bot 160: 1684–1696Google Scholar
  15. Lindsey K, Yeoman MM, Black GM, Mavituna F (1983) FEBS Lett 155: 143–149Google Scholar
  16. Mavituna F, Wilkinson AK, Williams PD (1987) In: Verrall MS, Hudson MJ (eds) Separations for Biotechnology. Ellis Hordwood, Chichester. pp 333–340Google Scholar
  17. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497Google Scholar
  18. Ochoa-Alejo N, García-Bautista (1989) Turrialba (In press)Google Scholar
  19. Palmer JE, Widholm J (1975) Plant Physiol 56: 233–238Google Scholar
  20. Quesnel AA, Ellis BE (1987) Plant Sci 49: 223–229Google Scholar
  21. Salgado-Garciglia R, López-Gutiérrez F, Ochoa-Alejo N (1985) Plant Cell, Tissue and Organ Culture 5: 3–12Google Scholar
  22. Smith PG, Villalon B, Villa PL (1987) HortScience 22: 11–13Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • R. Salgado-Garciglia
    • 1
  • N. Ochoa-Alejo
    • 1
  1. 1.Centro de Investigación y de Estudios Avanzados del IPN-Unidad IrapuatoIrapuato, Gto.Mexico

Personalised recommendations