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Plant Cell Reports

, Volume 8, Issue 11, pp 643–646 | Cite as

In vitro study of transgenic tobacco expressing Arabidopsis wild type and mutant acetohydroxyacid synthase genes

  • Pierre J. Charest
  • Jiro Hattori
  • Janice DeMoor
  • V. N. Iyer
  • Brian L. Miki
Article

Summary

Genes coding for the enzyme acetohydroxyacid synthase, often referred to as acetolactate synthase (AHAS, ALS; EC 4.1.3.18), from wild type Arabidopsis thaliana and a sulfonylurea-resistant mutant line GH50 (csrl-1; Haughn et al. 1988) were introduced in Nicotiana tabacum. Both genes were expressed at high levels with the 35S promoter. The csrl-1 gene conferred high levels of resistance to chlorsulfuron whereas the wild type gene did not. As selectable markers, chimaeric AHAS genes yielded transgenic plants on chlorsulfuron but at much lower efficiencies than with a chimaeric neomycin phosphotransferase gene on kanamycin (Sanders et al. 1987). Shoot differentiation from leaf discs was delayed on chlorsulfuron by 4–6 weeks. This study indicated a role for mutant AHAS genes in the genetic manipulation of herbicide resistance in transgenic plants but as selectable markers for plant cells undergoing differentiation no advantage over other genes was perceived.

Key words

Transgenic Acetohydroxyacid synthase Sulfonylurea Imidazolinone Selection 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Pierre J. Charest
    • 1
  • Jiro Hattori
    • 2
  • Janice DeMoor
    • 1
  • V. N. Iyer
    • 1
  • Brian L. Miki
    • 2
  1. 1.Department of BiologyCarleton UniversityOttawaCanada
  2. 2.Plant Research CentreAgriculture CanadaOttawaCanada

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