Plant Cell Reports

, Volume 4, Issue 6, pp 351–354 | Cite as

A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain)

  • Nirmalya Banerjee
  • Edmond de Langhe
Article
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Abstract

A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions is presented in this paper. Shoot-tip cultures of Musa cultivars (both banana and plantain) are induced by culturing small excised shoot apices on modified MS semisolid medium supplemented with various concentrations and combinations of auxins and cytokinins. The effects of cytokinin concentration in the medium as well as the genotypic configuration of the cultivars on the rate of shoot-bud proliferation have been tested. The established shoot-tip cultures grown on modified MS semisolid medium supplemented with IAA (0.18 mg/l) and BA (2.30 mg/l) have been successfully stored at 15°C with 1000 lux light intensity up to 13–17 months depending on the cultivar. The cultivars tested in the present investigation seem to vary in their ability to withstand minimal growth temperature.

Keywords

Tissue Culture Light Intensity Growth Temperature Clonal Propagation Shoot Apex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BA

Benzyladenine

IAA

Indoleacetic acid

IBA

Indolebutyric acid

MS

Murashige and Skoog

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Nirmalya Banerjee
    • 1
  • Edmond de Langhe
    • 1
  1. 1.Laboratorium voor Teeltfysiologie en Tropische FytotechnieKatholieke Universiteit LeuvenLeuvenBelgium

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