Molecular and General Genetics MGG

, Volume 225, Issue 2, pp 320–330 | Cite as

Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14

  • Wim G. Meijer
  • Annika C. Arnberg
  • Hans G. Enequist
  • Peter Terpstra
  • Mary E. Lidstrom
  • Lubbert Dijkhuizen
Article

Summary

The genes encoding the large (cfxL) and small (cfxS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisC/O) from Xanthobacter favus H4-14 were identified and characterized. The RuBisC/O genes are separated by 11 by and cotranscribed in Escherichia coli from the lac promoter in the order cfxLS. Primer extension and R-loop experiments with RNA isolated from autotrophically grown X. favus H4-14 showed that transcription of cfxL and cfxS initiated 22 by upstream from cfxL and resulted in a mRNA of at least 2.3 kb. DNA sequence analysis identified the start of an open reading frame transcribed divergently from cfxL, and displaying significant similarities with genes belonging to the lysR family of transcriptional activators. Downstream from cfxS an additional open reading frame was identified with unknown function. Expression studies showed that the genes encoding fructosebisphosphatase (cfxF) and phosphoribulokinase (cfxP) are located downstream from cfxLS. The cfxF and cfxP genes are cotranscribed in the same direction as cfxLS in the order cfxFP.

Key words

Ribulose-1,5-bisphosphate carboxylase Fructosebisphosphatase Phosphoribulokinase Sequence analysis Xanthobacter favus H4-14 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Wim G. Meijer
    • 1
  • Annika C. Arnberg
    • 2
  • Hans G. Enequist
    • 2
  • Peter Terpstra
    • 2
  • Mary E. Lidstrom
    • 3
  • Lubbert Dijkhuizen
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands
  2. 2.Department of BiochemistryUniversity of GroningenGroningenThe Netherlands
  3. 3.Keck Laboratories 138-78California Institute of TechnologyPasadenaUSA

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