Plant Cell Reports

, Volume 6, Issue 5, pp 341–344 | Cite as

Sequential rooting media and rooting capacity of Sequoiadendron giganteum in vitro. Peroxidase activity as a marker

  • J. Y. Berthon
  • N. Boyer
  • Th. Gaspar
Article
Received:
Revised:

Abstract

The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Peroxidase activity of all those cuttings on NAA+D2 first increased during the 7–9 first days and decreased in the days after. Rooting was obtained by transfer of the cuttings after periods longer than 7–9 days from the NAA+D2 inductive medium to a basal medium supplemented or not with rutin (expressive medium). The rooting capacity was emphasized by rutin treatment and was in correlation with the peroxidase peak reached on the NAA+D2 medium. Seedlings, characterised by the highest peroxidase activity, were most performing in rooting.

Keywords

Basal Medium Peroxidase Activity Rutin Inductive Medium High Peroxidase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BM

basal medium

D2

ergocalciferol

NAA

naphtaleneacetic acid

This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boyer N, Gaspar Th, Lamond M (1979) Z Pflanzenphysiol 93: 459–470Google Scholar
  2. Darimont E, Gaspar Th (1972) Soc bot France, Mémoires 1972 (coll. Morphologie) 211–222Google Scholar
  3. Druart Ph, Kevers C, Boxus Ph, Gaspar Th (1982) Z Pflanzenphysiol 108: 429–436Google Scholar
  4. Fouret Y, Arnaud Y, Maldiney R, Sotta B, Miginiac E (1986) CR Acad Sc Paris 303, série III: 135–138Google Scholar
  5. Franclet A (1981 a) In: AFOCEL (ed) Colloque International sur la Culture In Vitro des Essences Forestières, Imprimerie Couesnon, Champagne-sur-Seine, pp 55–64Google Scholar
  6. Franclet A (1981 b) In: AFOCEL Informations Forêt 165: 1–15Google Scholar
  7. Gaspar Th (1981) In: Jeffcoat B (ed) Aspects and Prospects of Plant Growth Regulators, British Plant Growth Regulator Group, Wantage, pp 39–49Google Scholar
  8. Gaspar Th, Penel C, Thorpe T, Greppin H (1982) Peroxidases 1970–1980. A survey of their biochemical and physiological roles in higher plants, Gaspar Th, Penel C, Thorpe T, Greppin H (ed), Université de Genève, GenèveGoogle Scholar
  9. Gaspar Th, Coumans M. (1987) In: Bonga JM, Durzan DJ (ed) Cell and Tissue Culture in Forestry, Martinus Nijhoff, Dordrecht, pp 202–217Google Scholar
  10. Gaspar Th, Hofinger M (1987) In: Davis TD, Sankhla N, Haissig B (ed) Adventitious Root Formation on Cuttings. In pressGoogle Scholar
  11. Haissig B.C. (1986) In: Jackson MB (ed) New Root Formation in Plants and Cuttings, Martinus Nijhoff, Dordrecht, pp 141–189Google Scholar
  12. Jarvis BC (1986) In: Jackson MB (ed) New Root Formation in Plants and Cuttings, Martinus Nijhoff, Dordrecht, pp 191–222Google Scholar
  13. Laemmli V (1970) Nature 227: 680–685Google Scholar
  14. Moncousin C, Gaspar Th (1983) Biochem Physiol Pflanzen 178: 263–271Google Scholar
  15. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497Google Scholar
  16. Spector T (1978) Anal Biochem 86: 142–146Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • J. Y. Berthon
    • 1
  • N. Boyer
    • 1
  • Th. Gaspar
    • 2
  1. 1.Laboratoire de PhytomorphogenèseUniversité de Clermont IIClermont-FerrandFrance
  2. 2.Hormonologie fondamentale et appliquée, Institut de Botanique B 22Université de Liège Sart TilmanLiègeBelgium

Personalised recommendations