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Molecular and General Genetics MGG

, Volume 126, Issue 4, pp 319–324 | Cite as

Evidence that pretreatment of Escherichia coli cells with N-methyl-N′-nitro-nitrosoguanidine enhances mutability of subsequently infecting phage λ

  • Sohei Kondo
  • Haruko Ichikawa
Article

Summary

The frequency of mutations in λ is significantly higher for host cells of a rec+ or recA- strain pretreated with N-methyl-N′-nitro-N-nitrosoguanidine (NG) than for untreated control cells. Direct NG treatment of λDNA-injected host cells results in about tenfold higher mutation frequency in λ than NG treatment of host cells alone. Since mutability of λ is enhanced by UV preirradiation of host cells in the rec+ strain but not in the recA- strain, indirect NG mutagenesis is different in mechanism from indirect UV mutagenesis. It is concluded that NG mutagenesis in λ consists of at least two processes: induction of rec+-independent mutagenic activity in the host bacterium and production of rec+-independent mutational damage to intracellular λDNA.

Keywords

Escherichia Coli Control Cell Host Cell Untreated Control Mutation Frequency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Sohei Kondo
    • 1
  • Haruko Ichikawa
    • 1
  1. 1.Department of Fundamental Radiology, Faculty of MedicineOsaka UniversityOsakaJapan

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