Isolation and characterization of a plaque-forming Lac-transducing phage Φ80plac with special reference to its integration and excision
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From a double lysogen for ϕ80dlac type II (Beckwith and Signer, 1966) and ϕ80, we isolated a plaque-forming lac-transducing coliphage ϕ80plac after selecting a strain with a suitable deletion in the ϕ80 prophage. The lac region of the phage is i+o+z+y+a- and supposed to be located between genes 15 (N) and imm (CI). The phage showed feckless phenotype indicating deletion of genes of the red system. The phage is also deleted for int or att function, and integrates exclusively at the host lac region, largely dependent on the host rec system. Excision of the prophage upon UV-irradiation or by mating the male lysogen with a non-lysogenic female was efficient and largely dependent on the host rec system. But a considerable amount of rec-independent excision was observed at least in the case of zygotic induction, which was not likely to be caused by int-xis, red or ter system of the phage. ϕ80plac/oe phage was also isolated by incorporation of oe1 mutation from strain 2000oe.
KeywordsSpecial Reference Strain 2000oe Zygotic Induction
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