Plant Cell Reports

, Volume 4, Issue 5, pp 237–240 | Cite as

Correlation of the appearance of morphinan alkaloids and laticifer cells in germinating Papaver bracteatum seedlings

  • Michael D. Rush
  • Toni M. Kutchan
  • Carmine J. Coscia


The course of alkaloid accumulation and laticifer cell appearance was compared in germinating P. bracteatum seedlings. Seedlings of various ages (0–14 days old) were analyzed for their dopamine, thebaine, morphinan alkaloid immunoreactivity, and benzophenanthridine alkaloid levels. Simultaneous electron microscopic studies revealed that seedlings were devoid of laticifer initials until day 3, where-upon their numbers increased with time. The appearance of appreciable amounts of thebaine only occurred after day 4 of germination. Conversely, dopamine was rapidly formed at the onset of germination and reached millimolar concentrations well before laticifer cells were detected. Benzophenanthridine alkaloid levels remained fairly constant over the period analyzed. These results support the theory that the presence of laticifer cells is necessary for the accumulation of morphinan but neither benzophenanthridine alkaloids nor their mutual precursor, dopamine.





  1. Böhm, H. (1981) Pharmazie 36: 660–667.Google Scholar
  2. Fairbairn, J.W. and El Masry, S. (1968) Phytochemistry 7, 181–187.Google Scholar
  3. Grove, M.D., Spencer, G.F., Wakeman, J.V. and Tookey, H.L. (1976) J. Agric. Food Chem. 24, 896–897Google Scholar
  4. Herbert, R.B. (1981) The Biosynthesis of Secondary Metabolites. Chapman and Hall, Ltd., London, New York.Google Scholar
  5. Ikuta, A., Syono, K. and Furuya, T. (1974) Phytochemistry 13: 2175–2179.Google Scholar
  6. Kamo, K.K. and Mahlberg, P.G. (1984) Journal of Natural Products 47: 682–686.Google Scholar
  7. Ketenes-Van Den Bosch, J.J., Salemink, C.A. and Khan, I. (1981) Journal of Ethnopharmacology 3: 21–38.Google Scholar
  8. Kutchan, T.M., Ayabe, S. and Coscia, C.J. (1985) The Chemistry and Biology of Isoquinoline Alkaloids. pp. 282–294, Springer Verlag, Berlin.Google Scholar
  9. Kutchan, T.M., Ayabe, S., Krueger, R.J., Coscia, E.M. and Coscia, C.J. (1983) Plant Cell Reports 2:281–284.Google Scholar
  10. Nessler, C.L. and Mahlberg, P.G. (1977) Amer. J. Bot. 64, 541–551.Google Scholar
  11. Nessler, C.L. and Mahlberg, P.G. (1978) Amer. J. Bot. 65, 978–983.Google Scholar
  12. Sarkany, S., Fridvalstky, L., Lovas, B., Verzar-Petri, G. (1964) 3rd Eur. Reg. Conf. Electron Microscopy. pp. 161–162.Google Scholar
  13. Shamma, M. and Moniot, J.L. (1978) Isoquinoline Alkaloids Research 1972–1977, Plenum Press, New York.Google Scholar
  14. Spenser, I.D. (1969) Comprehensive Biochemistry 20: 231–413.Google Scholar
  15. Thorpe, T.A. (1980) Internat. Rev. Cyt. 11A: 71–111.Google Scholar
  16. Thureson-Klein, A. (1970) Ann. Bot. 34, 751–759.Google Scholar
  17. Williams, M.A. (1977) Practical Methods in Electron Microscopy. pp. 33–38, North Holland Publishing Co., Amsterdam.Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Michael D. Rush
    • 1
  • Toni M. Kutchan
    • 1
  • Carmine J. Coscia
    • 1
  1. 1.E. A. Doisy Department of BiochemistrySt. Louis University School of MedicineSt. LouisUSA

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