Plant Cell Reports

, Volume 3, Issue 6, pp 254–257 | Cite as

Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures

  • K. Nakagawa
  • A. Konagai
  • H. Fukui
  • M. Tabata


Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400–800 mg/l) when 5–10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cells into the liquid medium, and an excess of berberine crystallized as its nitrate in the medium. When the cells were cultured in a modified LS medium containing 20 mM KNO3 and 40 mM NH4Cl in place of 20.6 mM NH4NO3 as nitrogen source, most of the alkaloid crystallized to form berberine chloride instead of nitrate. Minor alkaloids, thalifendine and magnoflorine, were also isolated from the medium and identified.


Alkaloid Liquid Medium Nitrogen Source NH4Cl Cell Suspension Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Linsmaier and Skoog (1965)


2,4-dichlorophenoxyacetic acid


1-naphthaleneacetic acid




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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • K. Nakagawa
    • 1
  • A. Konagai
    • 1
  • H. Fukui
    • 1
  • M. Tabata
    • 1
  1. 1.Faculty of Pharmaceutical SciencesKyoto UniversityKyotoJapan

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