Plant Cell Reports

, Volume 4, Issue 4, pp 220–223 | Cite as

Cultivation of cell cultures of Berberis wilsonae in 20-l airlift bioreactors

  • M. Breuling
  • A. W. Alfermann
  • E. Reinhard


Suspension cultures of Berberis wilsonae produce 4 berberine-type alkaloids: berberine, palmatine, columbamine and jatrorrhizine. In particular the formation of the phenolic alkaloids columbamine and jatrorrhizine and of berberine proves to be dependent on the concentration of dissolved oxygen. With higher aeration rates, berberine and jatrorrhizine yields can be increased considerably. Thus we reached an alkaloid yield of more than 3 g × 1−1 with 50% dissolved oxygen tension in the medium. As far as we know this is one of the best results in fermenting of alkaloid-producing cell cultures.


Oxygen Cell Culture Alkaloid Suspension Culture Oxygen Tension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



dissolved oxygen concentration in % saturation (using air)


high-performance liquid chromatography


volume air × volume medium−1 × minute−1


revolutions per minute


indole-3-acetic acid


2,4-dichlorophenoxy acetic acid


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Breuling
    • 1
  • A. W. Alfermann
    • 1
  • E. Reinhard
    • 1
  1. 1.Pharmazeutisches Institut (Pharmazeutische Biologie)Universität TübingenTübingenFRG

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