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Molecular and General Genetics MGG

, Volume 129, Issue 1, pp 61–75 | Cite as

Ribosomal protein in E. coli: Rate of synthesis and pool size at different growth rates

  • Kirsten Gausing
Article

Summary

Relative rates of ribosomal protein synthesis αr was determined by pulsechase labeling of cell protein followed by isolation of ribosomes by electrophoresis of complete lysates on agarose gels. The agarose gel fractionation of lysates is described in detail. Cells growing in acetate, glucose and enriched glucose media had αr values of 0.09, 0.16, and 0.24, respectively. Estimates of the free pool of ribosomal protein were obtained from the kinetics of pulse-chase labeling of ribosomal particles (including precursor particles) and gave maximal values of 1.1, 2.1, and 3.1% of total ribosomal protein at the three different growth rates. The kinetics indicate that the free concentrations in the cell are not the same for all ribosomal proteins.

Keywords

Fractionation Ribosomal Protein Relative Rate Cell Protein Pool Size 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Kirsten Gausing
    • 1
  1. 1.University Institute of MicrobiologyCopenhagenDenmark

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