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Plant Cell Reports

, Volume 5, Issue 2, pp 150–154 | Cite as

Plant regeneration by organogenesis in Glycine max

  • M. S. Wright
  • S. M. Koehler
  • M. A. Hinchee
  • M. G. Carnes
Article

Abstract

A procedure for the regeneration of fertile plants by organogenesis from tissue cultures of soybeans, Glycine max is described. Seeds were germinated on reduced inorganic salt MS medium containing 5μM BA. Cotyledonary nodes were excised and cultured on the same medium. Presence of BA in the medium during seed germination and culture of nodal explants was required for multiple shoot and shoot-bud formation. Histological analyses established the de novo nature of shoot regeneration. Separate reduction of the concentration of inorganic salts or substitution of sucrose for fructose during culture had minimal effects on the regeneration response. Conversely, if the BA was reduced, the inhibition response could not be overcome by increased salt concentration or altered carbon source.

Keywords

Fructose Plant Regeneration Inhibition Response Shoot Regeneration Inorganic Salt 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BA

benzyladenine

IAA

indoleacetic acid

SAS

secondary axillary shoots

MS

Murashige and Skoog (1962) medium

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • M. S. Wright
    • 1
  • S. M. Koehler
    • 1
  • M. A. Hinchee
    • 1
  • M. G. Carnes
    • 1
  1. 1.Monsanto Agricultural CompanySt. LouisUSA

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