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Histochemistry

, Volume 102, Issue 4, pp 323–327 | Cite as

Hybridocytochemical and immuno-ultrastructural study of calcitonin gene expression in cultured medullary carcinoma cells

  • M. Zabel
  • J. Seidel
  • J. Surdyk-Zasada
  • J. Grzeszkowiak
  • A. Kaczmarek
  • A. Górny
Original Paper

Abstract

The study was aimed at a morphological demonstration of calcitonin (CT) gene expression in cultured TT cells, or, more specifically, hybridocytochemical detection of CT mRNA and calcitonin gene-related peptide (CGRP) mRNA and ultrastructural localization of the two hormones. The TT cells originated from medullary carcinoma of human thyroid gland. Ultrastructural studies of TT cells demonstrated a well-developed rough endoplasmic reticulum, large Golgi apparatus and low number of secretory granules. Hybridocytochemical studies showed the presence of mRNAs for CT and CGRP in all TT cells. At the ultrastructural level, double immunolabelling demonstrated that the two hormones were always expressed together in the same secretory granules. Our results provide a significant addition to the biochemical studies performed up to now and indicate that all TT cells produce both mRNAs and both hormones in parallel.

Keywords

Calcitonin Thyroid Gland Golgi Apparatus Secretory Granule Rough Endoplasmic Reticulum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Verlag 1994

Authors and Affiliations

  • M. Zabel
    • 1
  • J. Seidel
    • 1
  • J. Surdyk-Zasada
    • 1
  • J. Grzeszkowiak
    • 1
  • A. Kaczmarek
    • 2
  • A. Górny
    • 2
  1. 1.Department of Histology and EmbryologyMedical AcademyPoznańPoland
  2. 2.Regional Centre of OncologyPoznańPoland

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