Histochemistry

, Volume 102, Issue 3, pp 173–176 | Cite as

Detection of manganese superoxide dismutase mRNA in the theca interna cells of rat ovary during the ovulatory process by in situ hybridization

  • J. Sasaki
  • T. Nomura
  • H. Mori
  • S. Watanabe
  • S. Kanda
  • E. F. Sato
  • M. Inoue
  • H. Watanabe
  • K. Utsumi
Original Paper
Accepted:

Abstract

To determine the possible involvement of reactive oxygen species in ovulation, dynamic aspects of superoxide dismutase (SOD) isozyme were studied in the ovaries of rats by in situ hybridization histochemistry. Previously, mRNA levels of ovarian manganese superoxide dismutase (Mn-SOD) were reported markedly to increase whilst enzymic activity of Mn-SOD decreased during the ovulatory process after treating immature rats with 10 and 5 Units, respectively, of pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG). Levels of Cu/Zn-SOD activity and Cu/Zn-SOD mRNA were reported to remain unchanged throughout ovulation. This increase in the Mn-SOD mRNA level was shown in the present study by in situ hybridization to be localized to the theca interna cells throughout the PMSG/HCG-induced ovulatory process. The observations suggest that the turnover rate of Mn-SOD but not Cu/Zn-SOD increases specifically in the mitochondria of these cells. SOD has been postulated to play important roles in steroidogenesis. The relationship is discussed between mitochondrial functions in steroid-secreting cells and superoxide radicals and related metabolite(s).

Keywords

Reactive Oxygen Species mRNA Level Superoxide Dismutase Mitochondrial Function Superoxide Radical 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Verlag 1994

Authors and Affiliations

  • J. Sasaki
    • 1
  • T. Nomura
    • 1
  • H. Mori
    • 1
  • S. Watanabe
    • 1
  • S. Kanda
    • 1
  • E. F. Sato
    • 2
  • M. Inoue
    • 2
  • H. Watanabe
    • 3
  • K. Utsumi
    • 4
  1. 1.Department of AnatomyOkayama University Medical SchoolOkayamaJapan
  2. 2.Department of BiochemistryOsaka City University Medical SchoolOsakaJapan
  3. 3.Department of Ultrastructural ScienceTokyo Dental CollegeChibaJapan
  4. 4.Center for Adult DiseasesKurashikiJapan

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