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Molecular and General Genetics MGG

, Volume 183, Issue 3, pp 437–439 | Cite as

Extensive rearrangements in the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana

  • Ferene Nagy
  • István Török
  • Pál Maliga
Article

Summary

Mitochondrial DNA (mtDNA) was studied in the leaves of Nicotiana tabacum+Nicotiana knightiana somatic hybrids previously described. Restriction patterns generated by the SalI and BamHI restriction endonucleases were different from both parents in the eight hybrids, and made up of parental and non-parental fragments. Rearrangements in the mtDNAs have been confirmed by DNA-DNA hybridization using, as a probe, labelled 2/12 plasmid DNA which contains the E. coli 16S and 23S rRNA genes. Novel patterns can be explained by new combinations of unaltered parental mtDNA molecules, and by genetic recombination.

Keywords

Recombination Restriction Endonuclease Somatic Hybrid Nicotiana Tabacum Restriction Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Arrand JR, Myers PA, Roberts RS (1978) A new restriction endonuclease from Streptomyces albus G. J Mol Biol 118:127–135Google Scholar
  2. Aviv D, Fluhr R, Edelman M, Galun E (1980) Progeny analysis of the interspecific somatic hybrids: Nicotiana tabacum (CMS) + Nicotiana sylvestris with respect to nuclear and chlorplast markers. Theor Appl Genet 56:145–150Google Scholar
  3. Aviv D, Galun E (1980) Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana sylvestris by fusion with X-irradiated N. tabacum protoplasts. Theor Appl Genet 58:121–127Google Scholar
  4. Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403Google Scholar
  5. Chen K, Wildman SG, Smith HH (1977) Chloroplast DNA distribution in parasexual hybrids as shown by polypeptide composition of fraction I protein. Proc Natl Acad Sci USA 74:5109–5112Google Scholar
  6. Gengenbach BG, Connely JA, Pring DR, Conde MF (1981) Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor Appl Genet 59:161–167Google Scholar
  7. Gillham NW (1978) Organelle heredity. Raven Press, New YorkGoogle Scholar
  8. Gleba YY, Butenko RH, Sytnik KM (1975) Protoplast fusion and parasexual hybridization in Nicotiana tabacum (in Russian) Dokl Acad Nauk USSR 221:1196–1198Google Scholar
  9. Jeffreys SAJ, Flavell RA (1977) A physical map of the DNA regions flanking the rabbit globin gene. Cell 12:429–439Google Scholar
  10. Kiss A, Sain B, Kiss I, Boros I, Udvardy A, Venetianer P (1978) Cloning of an E. coli ribosomal RNA gene and its promoter region from λ rifd 18. Gene 4:137–152Google Scholar
  11. Koncz Cs, Sain B (1980) Homology between rRNA of E. coli and mitochondrial DNA of maize. FEBS Lett 109:141–144Google Scholar
  12. Leaver J (1980) Mitochondrial genes and male sterility in plants. TIBS 58:248–252Google Scholar
  13. Levings CS, Kim BD, Pring DR, Conde MR, Mans RJ, Laughnan JR, Gabay-Laughnan SJ (1980) Cytoplasmic reversion of CmS-S in maize: Associations with a transpositional event. Science 209:1021–1023Google Scholar
  14. Melchers G, Scaristán MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplast. Carlsberg Res Comm 43:203–218Google Scholar
  15. Menczel L, Nagy F, Kiss Zs, Maliga P (1981) Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: Correlation of resistance to N. tabacum plasmids. Theor Appl Genet 59:191–195Google Scholar
  16. Quetier F, Vedel F (1977) Heterogeneous population of mitochondrial DNA molecules in higher plants. Nature 268:365–368Google Scholar
  17. Sidorov VA, Menczel L, Nagy F, Maliga P (1981) Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts. Planta 152:341–345Google Scholar
  18. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517Google Scholar
  19. Sparks RB, Dale RMK (1980) Characterization on H3-labelled supercoiled mitochondrial DNA from tobacco suspension culture cells. Mol Gen Genet 180:351–355Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Ferene Nagy
    • 1
  • István Török
    • 2
  • Pál Maliga
    • 1
  1. 1.Institute of Plant Physiology, Biological Research CenterHungarian Academy of SciencesSzegedHungary
  2. 2.Institute of Biochemistry, Biological Research CenterHungarian Academy of SciencesSzegedHungary

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