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Molecular and General Genetics MGG

, Volume 173, Issue 3, pp 263–269 | Cite as

Arginine catabolism: A new function of both octopine and nopaline Ti-plasmids of Agrobacterium

  • Jeffrey G. Ellis
  • Allen Kerr
  • Jacques Tempé
  • Annik Petit
Article

Summary

The oncogenic plasmids of Agrobacterium, the Ti-plasmids, carry genes that enable their bacterial host to catabolize opines. Opines are unusual amino acid derivatives that are only produced in crown gall tumours incited by oncogenic strains of Agrobacterium. The 2 opines, octopine and nopaline, are degraded by Agrobacterium strains carrying the octopine or the nopoline Ti-plasmid, respectively, to arginine and pyruvic acid, and to arginine and α-ketoglutaric acid. In this paper it is shown that the Ti-plasmids carry gene(s) involved in the utilisation of arginine as a carbon source. Strains harbouring wild type octopine or nopaline Ti-plasmids in the chromosomal context of strain C58C1 do not grow on arginine as a carbon source. However, they are able to grow on arginine provided that they are induced, or constitutive for opine catabolism. The features of ornithine utilisation are identical. The gene(s) involved in arginine and ornithine utilization in C58C1 (pTi-oct) or C58C1 (pTi-nop) are under the control of the regulator gene that controls octopine or nopaline catabolism. A tentative pathway of octopine utilization is proposed, in which at least two steps are Ti-plasmid coded, and probably belong to the same operon: 1-scission of octopine into arginine and pyruvic acid 2-transformation of an arginine derivative (GSA?) to glutamic acid.

Arginine utilization as a carbon source is therefore a new function of the Ti-plasmid. As this function is not inducible by arginine but by opines, it provides a method for selecting regulatory mutants of opine catabolism in the genetic background of strain C58.

Keywords

Arginine Ornithine Agrobacterium Pyruvic Acid Amino Acid Derivative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Jeffrey G. Ellis
    • 1
  • Allen Kerr
    • 1
  • Jacques Tempé
    • 2
  • Annik Petit
    • 2
  1. 1.Watte Agricultural Research InstituteGlen Osmond
  2. 2.Station de Génétique et d'Amélioration des PlantesINRAVersaillesFrance

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