Ribosomal protein neighborhoods
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Diimidoesters have been used to cross-link neighboring proteins in the 30S ribosomal subunit of Escherichia coli. Two pairs of cross-linked proteins have been identified by several independent techniques; these are S7-S9 and S13-S19. Since it has been shown earlier that these four proteins cooperate during ribosome assembly in vitro (Green and Kurland, 1973), their identification as near neighbors in the ribosome is quite suggestive. Thus, the data indicate that a cluster of proteins which interact with each other during assembly would be, in general, found in close proximity in the functional ribosome. A more detailed discussion of the arrangement of the proteins in the 30S subunit is presented.
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