Molecular and General Genetics MGG

, Volume 135, Issue 3, pp 273–276 | Cite as

The isolation, regeneration and fusion of Phycomyces protoplasts

  • Horst Binding
  • Hans Jürgen Weber
Short Communication


Germlings of Phycomyces blakesleeanus were converted to protoplasts using an enzyme solution of chitinase and chitosanase. The protoplasts were regenerated to normal growing mycelia. Protoplasts of different auxotrophic strains fused in diluted natural seawater and in Ca(NO3)2 solutions. 36 prototrophic strains were isolated (Table 1). Their heterokaryotic nature was proved by the segregation of the markers in vegetative spores.


Enzyme Chitinase Enzyme Solution Natural Seawater Phycomyces 
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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Horst Binding
    • 1
  • Hans Jürgen Weber
    • 2
  1. 1.Abteilung StraubMax-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut)Köln
  2. 2.Division of BiologyCalifornia Institute of TechnologyPasadena

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