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Molecular and General Genetics MGG

, Volume 135, Issue 3, pp 273–276 | Cite as

The isolation, regeneration and fusion of Phycomyces protoplasts

  • Horst Binding
  • Hans Jürgen Weber
Short Communication

Summary

Germlings of Phycomyces blakesleeanus were converted to protoplasts using an enzyme solution of chitinase and chitosanase. The protoplasts were regenerated to normal growing mycelia. Protoplasts of different auxotrophic strains fused in diluted natural seawater and in Ca(NO3)2 solutions. 36 prototrophic strains were isolated (Table 1). Their heterokaryotic nature was proved by the segregation of the markers in vegetative spores.

Keywords

Enzyme Chitinase Enzyme Solution Natural Seawater Phycomyces 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Binding, H.: Fusion experiments with isolated protoplasts of Petunia hybrida L. Z. Pflanzenphysiol. 72, 422–426 (1974)CrossRefGoogle Scholar
  2. Ferenczy, L., Kevei, F., Zsolt, J.: Fusion of fungal protoplasts. Nature (Lond.) 248, 793–794 (1974)CrossRefGoogle Scholar
  3. Heisenberg, M., Cerdá-Olmedo, E.: Segregation of heterokaryons in the asexual cycle of Phycomyces. Molec. gen. Genet. 102, 187–195 (1968)CrossRefGoogle Scholar
  4. Kao, K. N., Michayluk, M. R.: A method for high frequency intergeneric fusion of plant protoplasts. Planta (Berl.) 115, 355–367 (1974)CrossRefGoogle Scholar
  5. Keller, W. A., Melchers, G.: The effect of high pH and calcium on tobacco leaf protoplast fusion. Z. Naturforsch. 28c, 737–741 (1973)CrossRefGoogle Scholar
  6. Melchers, G.: Genetik und Pflanzenzüchtung mit mikrobiologischen Methoden. Stuttgart: Planta Medica, Hippokrates-Verlag (in press)Google Scholar
  7. Melchers, G.: Summation: Haploid research in higher plants. Proc. Internat. Symp. “Haploids in higher plants — Advances and Potencial”, June 1974, Crop Science Dept., Univers. of Guelph, Can. ed. K. J. KashaGoogle Scholar
  8. Melchers, G., Keller, W.A., Labib, G.: The consequence of the fusion of mesophyll protoplasts. Abstr. 3. Internat. Congr. of plant tissue and cell culture, July 1974, Leicester, in press (1974a)Google Scholar
  9. Melchers, G., Keller, W.A., Labib, G.: Somatic hybridyzation. Proc. II. John Innes Symp., Juli 1974, Norwich (1974b)Google Scholar
  10. Price, J. S., Storck, R.: Induction in Streptomyces of the synthesis of an extracellular chitosanase lysing Mucor cell walls. Abstr. Ann. Meet. Amer. Soc. of Microbiol. 146 (1974)Google Scholar
  11. Schieder, O.: Fusionen zwischen Protiplasten von Sphaerocarpos donnellii Aust.-Mutanten. Biochemie u. Physiol. d. Pflanzen 165, 433–435 (1974a)CrossRefGoogle Scholar
  12. Schieder, O.: Selektion einer somatischen Hybriden nach Fusion von Protoplasten auxotropher Mutanten von Sphaerocarpos donnellii Aust. Z. Pflanzenphysiol. 74, 357–365 (1974)CrossRefGoogle Scholar
  13. Sutter, R. P.; Mutations effecting sexual development in Phycomyces blakesleeanus (in press). Proc. Nat. Acad. Sci., USA. (in press)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Horst Binding
    • 1
  • Hans Jürgen Weber
    • 2
  1. 1.Abteilung StraubMax-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut)Köln
  2. 2.Division of BiologyCalifornia Institute of TechnologyPasadena

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