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Molecular and General Genetics MGG

, Volume 135, Issue 3, pp 213–229 | Cite as

Etude d'un mutant bobbed ayant une quantité normale d'ADN ribosomal

  • M. Marrakechi
Article

Study of a non deleted bobbed mutant

Summary

We studied a bobbed mutant of Drosophila melanogaster whose rDNA content is equal to that of a wild type strain. Biochemical studies showed the following results:
  • -The rDNA content is the same in ovaries and carcasses. This implies a lack of specific rDNA amplification in the ovaries.

  • -In vitro and In vivo studies indicate that on the whole the rate of rRNA synthesis is not decreased.

  • -A specific degradation of the 28s species seems to occur during the precursors processing. This result could be explained by a qualitative defect in the rDNA transcripts.

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Bibliographie

  1. Bernardi, G.: Chromatography of nucleic acids on hydroxyapatite. I. Chromatography of native DNA. Biochem. biophys. Acta (Amst.) 174, 423–434 (1969)CrossRefGoogle Scholar
  2. Callan, H. G.: On the organization of genetic units in chromosomes J. Cell Sci. 2, 1–7 (1967)PubMedGoogle Scholar
  3. Evans, B. B., Gall J. G.: Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis. Biochem. biophys. Acta (Amst.) 166 247–276 (1968)Google Scholar
  4. Gall, J. G., Mac Gregor, H. C., Kidston, M. E.: Gene amplification in the oocytes of Dysticus water beetles. Chromosoma (Berl.) 26, 169–187 (1969)CrossRefGoogle Scholar
  5. Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol. 12, 829–842 (1965)CrossRefGoogle Scholar
  6. Greenberg, J. R.: Synthesis and properties of ribosomal RNA in Drosophila. J. molec. Biol. 46, 85–98 (1969)CrossRefGoogle Scholar
  7. Henning, W., Meer, B.: Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature (Lond.) New Biol. 233, 70–71 (1971)CrossRefGoogle Scholar
  8. Kumar, A.: Ribosome synthesis in Tetrahymena pyriformis. J. Cell Biol. 45, 623–634 (1970)CrossRefGoogle Scholar
  9. Lima de Faria, A., Birnstiel, M., Jaworska, H.: Amplification of ribosomal cistrons in the heterochromatin of acheta. Genetics 61, Suppl. 1, 145–159 (1969)Google Scholar
  10. Loening, U. E.: The fractionation of High-molecular weight RNA by polyacrylamide gel electrophoresis. J. Biochem. 102, 252 (1967)CrossRefGoogle Scholar
  11. Marrakechi, M., Prud'homme, N.: A study of bobbed mutants induced by ethyl methane sulfonate in Drosophila melanogaster. Biochem. biophys. Res. Commun. 43, 273–277 (1971)CrossRefGoogle Scholar
  12. Mazabraud, A.: Etude des ARN et de leur métabolisme dans les ovaires de Drosophila melanogaster. Thèse de Illème Cycle Paris (1973)Google Scholar
  13. Mohan, J., Ritossa, F. M.: Regulation of ribosomal RNA synthesis and its bearing on the bobbed phénotype in Drosophila melanogaster. Develop. Biol. 22, 495–512 (1970)CrossRefGoogle Scholar
  14. Penman, S.: RNA metabolism in the HeLa cell nucleus. J. molec. Biol. 17, 117–130 (1966)CrossRefGoogle Scholar
  15. Petri, W. H., Fristrom, J. W., Stewart, D. J., Hanly, E. W.: The in vitro synthesis and characteristics of ribosomal RNA in imaginal discs of Drosophila melanogaster. Molec. Gen. Genet. 110, 245–262 (1971)CrossRefGoogle Scholar
  16. Quagliarotti, G., Ritossa, F. M.: On the arrangement of genes for 28s and 18s ribosomal RNA's in Drosophila melanogaster. J. molec. Biol. 36, 57–69 (1968)CrossRefGoogle Scholar
  17. Ritossa, F. M.: Nonoperative DNA complementary to ribosomal RNA. Proc. nat. Acad. Sci. (Wash.) 50, 1124–1131 (1968)CrossRefGoogle Scholar
  18. Ritossa, F. M., Atwood, K. C., Spiegelman, S.: A molecular explanation of bobbed mutants of Drosophila as partial deficiencies of “ribosomal” DNA Genetics 54, 819–834 (1966)PubMedPubMedCentralGoogle Scholar
  19. Ritossa, F. M., Spiegelman, S.: Localization of DNA complementary to ribosomal RNA in the nucleolus organizer regions of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 53, 737–745 (1965)CrossRefGoogle Scholar
  20. Scherrer, K., Darnell, J. E.: Sedimentation characteristics of rapidly labelled RNA from Hela cells. Biochem. biophys. Res. Commun. 7, 486–490 (1962)CrossRefGoogle Scholar
  21. Sillevis Smitt, W. W., Vlak, J. M., Shiphof, R., Rozijn, Th. H.: Precursors of ribosomal RNA in yeast nucleus. Exp. Cell Res. 71, 33–40 (1972)CrossRefGoogle Scholar
  22. Spear, B. B., Gall, J. G.: Independant control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 70, 1359–1363 (1973)CrossRefGoogle Scholar
  23. Yankofsky, S. A., Spiegelman, S.: Distincts cistrons for the two ribosomal RNA components. Proc. nat. Acad. Sci. (Wash.) 49, 538–544 (1963)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • M. Marrakechi
    • 1
  1. 1.Centre de Génétique Moléculaire du C.N.R.S.Gif s/YvetteFrance

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